Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
using 2.0 M ammonium sulfate and 0.2 M sodium acetate pH 4.5 | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
K35A | the mutation does not alter the oligomeric status or spectroscopic properties of the enzyme, however, NADPH binding is altered by ca 20fold with much less change (ca 4fold) in NADH binding compared to the wild type enzyme | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q5HG24 | - |
- |
Staphylococcus aureus COL | Q5HG24 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA affinity column chromatography and DEAE-Sepharose column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Staphylococcus aureus | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Staphylococcus aureus COL | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Staphylococcus aureus | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Staphylococcus aureus COL | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
DapB | - |
Staphylococcus aureus |
DHDPR | - |
Staphylococcus aureus |
dihydrodipicolinate reductase | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Staphylococcus aureus | |
NADPH | NADPH is a more preferred co-factor than NADH | Staphylococcus aureus |