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Literature summary for 1.16.98.B1 extracted from

  • Krompholz, N.; Krischkowski, C.; Reichmann, D.; Garbe-Schönberg, D.; Mendel, R.R.; Bittner, F.; Clement, B.; Havemeyer, A.
    The mitochondrial amidoxime reducing component (mARC) is involved in detoxification of N-hydroxylated base analogues (2012), Chem. Res. Toxicol., 25, 2443-2450 .
    View publication on PubMed
Search for more literature for 1.16.98.B1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 N4-hydroxycytidine pH 6.0, 37°C Homo sapiens
0.52
-
 N4-hydroxycytosine pH 6.0, 37°C Homo sapiens
0.72
-
 N6-hydroxyadenine pH 6.0, 37°C Homo sapiens
0.77
-
 N6-hydroxyadenine pH 6.0, 37°C Homo sapiens
0.98
-
 N6-hydroxyadenosine pH 6.0, 37°C Homo sapiens
1.5 2 N4-hydroxycytosine pH 6.0, 37°C Homo sapiens
5.28
-
 N6-hydroxyadenosine pH 6.0, 37°C Homo sapiens
5.65
-
 N4-hydroxycytidine pH 6.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5VT66 isoform mARC1
-
Homo sapiens Q969Z3 isoform mARC2
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
additional information activity is found in all tested tissues with the highest detectable conversion rates in liver, kidney, thyroid, and pancreas Homo sapiens
-
pancreas
-
 Homo sapiens
-
thyroid gland
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0396
-
 substrate N4-hydroxycytosine, pH 6.0, 37°C Homo sapiens
0.0502
-
 substrate N4-hydroxycytidine, pH 6.0, 37°C Homo sapiens
0.1031
-
 substrate N4-hydroxycytidine, pH 6.0, 37°C Homo sapiens
0.105
-
 substrate N6-hydroxyadenosine, pH 6.0, 37°C Homo sapiens
0.1189
-
 substrate N4-hydroxycytosine, pH 6.0, 37°C Homo sapiens
0.133
-
 substrate N6-hydroxyadenine, pH 6.0, 37°C Homo sapiens
0.142
-
 substrate benzamidoxime, pH 6.0, 37°C Homo sapiens
0.1594
-
 substrate benzamidoxime, pH 6.0, 37°C Homo sapiens
0.1808
-
 substrate N6-hydroxyadenine, pH 6.0, 37°C Homo sapiens
0.2202
-
 substrate N6-hydroxyadenosine, pH 6.0, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 [Fe(II)-cytochrome b5] + 2 H+ + benzamidoxime
-
 Homo sapiens 2 [Fe(III)-cytochrome b5] + H2O + benzamidine
-
 ?
2 [Fe(II)-cytochrome b5] + 2 H+ + N4-hydroxycytidine
-
 Homo sapiens 2 [Fe(III)-cytochrome b5] + H2O + cytidine
-
 ?
2 [Fe(II)-cytochrome b5] + 2 H+ + N4-hydroxycytosine
-
 Homo sapiens 2 [Fe(III)-cytochrome b5] + H2O + cytosine
-
 ?
2 [Fe(II)-cytochrome b5] + 2 H+ + N6-hydroxyadenine
-
 Homo sapiens 2 [Fe(III)-cytochrome b5] + H2O + adenine
-
 ?
2 [Fe(II)-cytochrome b5] + 2 H+ + N6-hydroxyadenosine
-
 Homo sapiens 2 [Fe(III)-cytochrome b5] + H2O + adenosine
-
 ?

General Information

General Information Comment Organism
metabolism both mARC isoforms are able to reduce N-hydroxylated base analogues, with mARC1 representing the more efficient isoform Homo sapiens