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Literature summary for 1.16.3.4 extracted from

  • Singh, S.K.; Grass, G.; Rensing, C.; Montfort, W.R.
    Cuprous oxidase activity of CueO from Escherichia coli (2004), J. Bacteriol., 186, 7815-7817 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
C500S mutant designed to incapacitate binding of the T1 copper, mutant protein does not support resistance to copper toxicity when placed in a cell line missing a functional CueO gene Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 Cu+ pH 7, presence of Cu(II), temperature not specified in the publication Escherichia coli
0.129
-
 Fe2+ pH 5, presence of Cu(II), temperature not specified in the publication Escherichia coli
0.165
-
 Cu+ pH 5, temperature not specified in the publication Escherichia coli
0.169
-
 Cu+ pH 5, presence of Cu(II), temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P36649
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 Cu+ + 4 H+ + O2
-
 Escherichia coli 4 Cu2+ + 2 H2O
-
 ?
4 Fe2+ + 4 H+ + O2 reaction of EC 1.16.3.1 Escherichia coli 4 Fe3+ + 2 H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.95
-
 Cu+ pH 7, presence of Cu(II), temperature not specified in the publication Escherichia coli
3.58
-
 Fe2+ pH 5, presence of Cu(II), temperature not specified in the publication Escherichia coli
10.85
-
 Cu+ pH 5, presence of Cu(II), temperature not specified in the publication Escherichia coli
15.23
-
 Cu+ pH 5, temperature not specified in the publication Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.5
-
 Cu+ pH 7, presence of Cu(II), temperature not specified in the publication Escherichia coli
28.3
-
 Fe2+ pH 5, presence of Cu(II), temperature not specified in the publication Escherichia coli
65
-
 Cu+ pH 5, presence of Cu(II), temperature not specified in the publication Escherichia coli
91.67
-
 Cu+ pH 5, temperature not specified in the publication Escherichia coli