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Literature summary for 1.16.3.4 extracted from
- A new multicopper oxidase from Gram-positive bacterium Rhodococcus erythropolis with activity modulating methionine rich tail (2013), Protein Expr. Purif., 89, 97-108.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| apo- and holo-form of CueO are expressed in Escherichia coli | Rhodococcus erythropolis |
| apo- and holo-form of CueO are expressed in Escherichia coli as His-tagged fusion proteins | Rhodococcus erythropolis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | truncated enzyme with a deleted methionine-rich C-terminal tail region shows a decreased turnover and a slightly lower Km value. Ki (Cu+) value increased compared to wild-type. Catalytic efficacy similar to wild-type | Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu+ | - |
Rhodococcus erythropolis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
Cu+ | pH 5.5, 30°C, mutant containing a deleted methionine-rich C-terminal tail | Rhodococcus erythropolis | |
| 0.00008 | - |
Cu+ | pH 5.5, 30°C, wild-type | Rhodococcus erythropolis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | upon incubation with Cu2+ ions, low active apo-CueOR is converted into the active holo-CueOR in vivo | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Rhodococcus erythropolis |
| 55000 | - |
SDS-PAGE, apo-CueO | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulphonic acid + Cu(I) + O2 | - |
Rhodococcus erythropolis | ? | - |
? | |
| 2,6-dimethoxyphenol + Cu(I) + O2 | - |
Rhodococcus erythropolis | ? | - |
? | |
| Cu+ + H+ + O2 | - |
Rhodococcus erythropolis | Cu2+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CueO | - |
Rhodococcus erythropolis |
| More | enzyme possesses EC 1.10.3.2 laccase activity | Rhodococcus erythropolis |
| multicopper oxidase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | holo CueO | Rhodococcus erythropolis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
Cu+ | pH 5.5, 30°C, mutant containing a deleted methionine-rich C-terminal tail | Rhodococcus erythropolis | |
| 0.155 | - |
Cu+ | pH 5.5, 30°C, wild-type | Rhodococcus erythropolis | |
| 22.15 | - |
2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulphonic acid | pH 5.5, 30°C | Rhodococcus erythropolis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Rhodococcus erythropolis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000039 | - |
Cu+ | pH 5.5, 30°C, wild-type | Rhodococcus erythropolis | |
| 0.00008 | - |
Cu+ | pH 5.5, 30°C, mutant containing a deleted methionine-rich C-terminal tail | Rhodococcus erythropolis | |
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