KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
NADH | pH 7.0, 85°C | Archaeoglobus fulgidus | |
0.066 | - |
Fe(III)-EDTA | pH 7.0, 85°C, cosubstrate: NADPH | Archaeoglobus fulgidus | |
0.08 | - |
NADPH | pH 7.0, 85°C | Archaeoglobus fulgidus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | high cellular abundance, 0.75% of the total soluble protein | Archaeoglobus fulgidus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require Mg2+ for optimal activity | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
2 * 18000, SDS-PAGE | Archaeoglobus fulgidus |
18659 | - |
2 * 18659, calculated from sequence | Archaeoglobus fulgidus |
40000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29428 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3503 | - |
pH 7.0, 85°C | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(III)-EDTA + NADH | no activity is obtained with uncomplexed Fe3+, i.e. FeCl3 and Fe(OH)3, with the menaquinone analog dimethylnaphthoquinone or with Fe2+-EDTA. The enzyme does not reduce EDTA-chelated Ag+ and Cu2+. With Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor | Archaeoglobus fulgidus | 2 Fe(II)-EDTA + NAD+ + H+ | - |
? | |
2 Fe(III)-EDTA + NADPH | no activity is obtained with uncomplexed Fe3+, i.e. FeCl3 and Fe(OH)3, with the menaquinone analog dimethylnaphthoquinone or with Fe2+-EDTA. The enzyme does not reduce EDTA-chelated Ag+ and Cu2+. With Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor | Archaeoglobus fulgidus | 2 Fe(II)-EDTA + NADP+ + H+ | - |
? | |
additional information | the ferric reductase also functions as an NAD(P)H:flavin oxidoreductase | Archaeoglobus fulgidus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 18000, SDS-PAGE | Archaeoglobus fulgidus |
homodimer | 2 * 18659, calculated from sequence | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
AF0830 | - |
Archaeoglobus fulgidus |
ferric reductase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Archaeoglobus fulgidus |
88 | - |
- |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | 50°C: about 50% of maximal activity, 100°C: about 50% of maximal activity | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 40% of maximal activity, pH 8.0: about 60% of maximal activity | Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein, enzyme bound flavin is involved in catalysis, contains 1.4 FMN per monomer, non-covalently bound to the enzyme | Archaeoglobus fulgidus | |
NADH | with Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor | Archaeoglobus fulgidus | |
NADPH | with Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor | Archaeoglobus fulgidus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Archaeoglobus fulgidus | calculated from sequence | - |
5.84 |