Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR enzyme expresion analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami | Sonneratia alba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a Fe-SOD | Sonneratia alba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Sonneratia alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sonneratia alba | - |
collected from Qinglangang Mangrove Reserve, Wenchang, Hainan, China | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-gami by nickel affinity chromatography | Sonneratia alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Sonneratia alba | - |
fruit | high expression level | Sonneratia alba | - |
leaf | high expression level | Sonneratia alba | - |
additional information | quantitative real-time PCR enzyme expresion analysis | Sonneratia alba | - |
root | low expression level | Sonneratia alba | - |
seedling | - |
Sonneratia alba | - |
stem | - |
Sonneratia alba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme activity is measured byy the enzyme caused inhibition of the xanthine oxidase coupling reaction | Sonneratia alba | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Sonneratia alba |
Synonyms | Comment | Organism |
---|---|---|
FeSOD | - |
Sonneratia alba |
iron superoxide dismutase | - |
Sonneratia alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sonneratia alba |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant His-tagged enzyme, 1 h, 78% activity remaining | Sonneratia alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Sonneratia alba |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | purified recombinant His-tagged enzyme, 25°C, 1 h, stable | Sonneratia alba |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sonneratia alba | sequence calculation | - |
6.42 |