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Literature summary for 1.14.99.58 extracted from
- Essential amino acid residues controlling the unique regioselectivity of heme oxygenase in Pseudomonas aeruginosa (2004), J. Am. Chem. Soc., 126, 4466-4467 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F189A | the heme degradation activity of the mutant is similar to that of the wild-type enzyme. The mutant produces only beta- and delta-biliverdins, but no alpha-biliverdin | Pseudomonas aeruginosa |
| F189G | the heme degradation activity of the mutant is similar to that of the wild-type enzyme. The mutant produces only beta- and delta-biliverdins, but no alpha-biliverdin | Pseudomonas aeruginosa |
| F189L | the heme degradation activity of the mutant is similar to that of the wild-type enzyme. The mutant produces only beta- and delta-biliverdins, but no alpha-biliverdin | Pseudomonas aeruginosa |
| F189T | the heme degradation activity of the mutant is similar to that of the wild-type enzyme. The mutant produces only beta- and delta-biliverdins, but no alpha-biliverdin | Pseudomonas aeruginosa |
| K132A | the heme degradation activity of the mutant is similar to that of the wild-type enzyme, but the mutant produces 12% of alpha-biliverdin in addition to the formation of normal beta- (26%) and delta- (62%) biliverdins | Pseudomonas aeruginosa |
| K34A | the heme degradation activity of the mutant is similar to that of the wild-type enzyme, but the mutant produces 11% of alpha-biliverdin in addition to the formation of normal beta- (34%) and delta- (55%) biliverdins | Pseudomonas aeruginosa |
| K34A/K132A | the heme degradation activity of the mutant is similar to that of the wild-type enzyme, but the mutant produces 18% of alpha-biliverdin in addition to the formation of normal beta- (27%) and delta- (56%) biliverdins | Pseudomonas aeruginosa |
| T189W | the heme degradation activity of the mutant is similar to that of the wild-type enzyme. The mutant produces only beta- and delta-biliverdins, but no alpha-biliverdin | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoheme + reduced acceptor + O2 | Pseudomonas aeruginosa | - |
biliverdin-IX-beta + CO + Fe2+ + acceptor + H2O | - |
? |  |
| protoheme + reduced acceptor + O2 | Pseudomonas aeruginosa | - |
biliverdin-IX-delta + CO + Fe2+ + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoheme + reduced acceptor + O2 | - |
Pseudomonas aeruginosa | biliverdin-IX-beta + CO + Fe2+ + acceptor + H2O | - |
? | |
| protoheme + reduced acceptor + O2 | - |
Pseudomonas aeruginosa | biliverdin-IX-delta + CO + Fe2+ + acceptor + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PigA | - |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Pseudomonas aeruginosa | |
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Release 2023.1 (January 2023)
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