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Literature summary for 1.14.99.55 extracted from
- A family of starch-active polysaccharide monooxygenases (2014), Proc. Natl. Acad. Sci. USA, 111, 13822-13827.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose dehydrogenase | cellobiose dehydrogenase may serve as the physiologic electron donor. In the presence of cellobiose dehydrogenase and cellobiose the enzyme activity is comparable to that with excess ascorbate. | Neurospora crassa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Neurospora crassa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | one copper atom per enzyme molecule | Neurospora crassa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
calculated from amino acid sequence | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + ascorbic acid + O2 | Neurospora crassa | - |
malto-aldonic acids + dehydroascorbate | - |
? | |
| starch + AH2 + O2 | Neurospora crassa | - |
aldonic acid-terminated malto-oligosaccharides + A + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | Q7SCE9 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| methylation | the enzyme contains a methylated N-terminal histidine | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column, followed by a size exclusion column | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + ascorbic acid + O2 | - |
Neurospora crassa | malto-aldonic acids + dehydroascorbate | - |
? | |
| starch + AH2 + O2 | - |
Neurospora crassa | aldonic acid-terminated malto-oligosaccharides + A + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LPMO | ambiguous | Neurospora crassa |
| More | in CAZY database classified as auxiliary activity family 13, AA13 | Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
assay at | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cellobiose dehydrogenase may serve as the physiologic electron donor. In the presence of cellobiose dehydrogenase and cellobiose the enzyme activity is comparable to that with excess ascorbate. | Neurospora crassa |
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Release 2023.1 (January 2023)
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