Cloned (Comment) | Organism |
---|---|
gene RebH, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lentzea aerocolonigenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + FADH2 + chloride + O2 + H+ | Lentzea aerocolonigenes | - |
7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentzea aerocolonigenes | Q8KHZ8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography | Lentzea aerocolonigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-5-amino-L-tryptophan + FAD + 2 H2O | - |
? | |
5-bromo-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-5-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
5-fluoro-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-5-fluoro-L-tryptophan + FAD + 2 H2O | - |
? | |
5-hydroxy-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-5-hydroxy-L-tryptophan + FAD + 2 H2O | - |
? | |
5-methyl-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-5-methyl-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
additional information | a combination of tryptophan synthase from Salmonella enterica and tryptophan-7-halogenase RebH from Lechevalieria aerocolonigenes for the regioselective halogenation of substituted tryptophan derivatives. An array of C5- and C6-substituted tryptophan derivatives is synthesized and 7-halogenated by RebH. Halogenation products of tryptophan derivatives by RebH, overview. Halogenation occurs in many cases preferably in the electronically unfavored C7 position, even in presence of deactivating ortho/para-directing groups like 5-fluoro | Lentzea aerocolonigenes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent halogenase | - |
Lentzea aerocolonigenes |
L-tryptophan-7-halogenase | - |
Lentzea aerocolonigenes |
RebH | - |
Lentzea aerocolonigenes |
Trp halogenase | - |
Lentzea aerocolonigenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lentzea aerocolonigenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Lentzea aerocolonigenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Lentzea aerocolonigenes |