Literature summary for 1.14.19.67 extracted from

Gesell, A.; Rolf, M.; Ziegler, J.; Diaz Chavez, M.L.; Huang, F.C.; Kutchan, T.M.
CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy (2009), J. Biol. Chem., 284, 24432-24442.

Search for more literature for 1.14.19.67

Cloned(Commentary)

Cloned (Comment)	Organism
full-length CYP719B1 cDNA is generated by amplification out of bacterial vector pCR2.1 and inserted into baculovirus transfer vector pVL1392	Papaver somniferum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0062	-	(R)-reticuline	-	Papaver somniferum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	determined by SDS-PAGE	Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2	Papaver somniferum	-	salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Papaver somniferum	B1NF18	-	-

Purification (Commentary)

Purification (Comment)	Organism
microsomes from insect cells are prepared	Papaver somniferum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2	-	Papaver somniferum	norsalutaridine + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2	-	Papaver somniferum	salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP719B1	-	Papaver somniferum
salutaridine synthase	-	Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay for kinetic analysis	Papaver somniferum
37	-	activity assay	Papaver somniferum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.027	-	(R)-reticuline	-	Papaver somniferum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	activity assay	Papaver somniferum
8	-	assay for kinetic analysis	Papaver somniferum

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Papaver somniferum

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Release 2023.1 (January 2023)