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Literature summary for 1.14.19.6 extracted from

  • Bielecka, M.; Kaminski, F.; Adams, I.; Poulson, H.; Sloan, R.; Li, Y.; Larson, T.R.; Winzer, T.; Graham, I.A.
    Targeted mutation of DELTA12 and DELTA15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil (2014), Plant Biotechnol. J., 12, 613-623 .
    View publication on PubMed
Search for more literature for 1.14.19.6

Cloned(Commentary)

Cloned (Comment) Organism
gene FAD2A, quantitative RT-PCR enzyme expression analysis, CSFAD2A is cloned into the expression vector pESCTRP containing the galactose-inducible GAL1 promoter, recombinant expression in Saccharomyces cerevisiae Cannabis sativa

Protein Variants

Protein Variants Comment Organism
additional information following four backcrosses and sibling crosses to achieve homozygosity, csfad2a-1 is grown in the field and produces a 70 molar per cent high oleic acid (18:1DELTA9) oil at yields similar to wild-type. Cold-pressed high oleic oil produces fewer volatiles and has a sevenfold increase in shelf life compared to wild-type. Two low abundance octadecadienoic acids, 18:2DELTA6,9 and 18:2DELTA9,15, are identified in the high oleic oil, and their presence suggests remaining endogenous desaturase activities utilize the increased levels of oleic acid as substrate. Dramatic fatty acid level changes observed in csfad2a-1 seed confirm that the predicted truncated CSFAD2A protein is nonfunctional. A second allele, csfad2a-2, which carries two point mutations giving rise to a proline to leucine transition at positions 218 and 375 of the predicted amino acid sequence of CSFAD2A. Homozygous csfad2a-2 (BC1F1) seed accumulate nearly 70 molar per cent of oleic acid, low levels of 18:2DELTA6,9 and 18:2DELTA9,15 and decreased levels of linoleic acid and alpha-linolenic acid compared to heterozygous and segregating wild-type seeds from the same population. This seed oil phenotype is very similar to that of csfad2a-1 and is consistent with one or both of the P to L transitions disrupting protein function Cannabis sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Cannabis sativa 5783
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membrane membrane-bound Cannabis sativa 16020
-
microsome
-
 Cannabis sativa
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ Cannabis sativa
-
 linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cannabis sativa A0A088MFF4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo high-level expression of CSFAD2A during embryo development Cannabis sativa
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additional information quantitative RT-PCR enzyme expression analysis Cannabis sativa
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seed developing Cannabis sativa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinantly expressed enzyme CSFAD2A is a DELTA12 desaturase and has DELTAx+3 activity Cannabis sativa ?
-
 ?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Cannabis sativa linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
CSFAD2A
-
 Cannabis sativa
DELTA 12 desaturase
-
 Cannabis sativa
DELTA12 desaturase
-
 Cannabis sativa
FAD2A
-
 Cannabis sativa

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
 Cannabis sativa

General Information

General Information Comment Organism
evolution all members of the CSFAD2 and CSD6 subclasses of microsomal desaturases consist of a single exon. Evolutionary relationship of Cannabis sativa putative desaturases, overview Cannabis sativa
additional information hemp seeds contain seven DELTA12 desaturases, FAD2A is the major DELAT12 desaturase in hemp seed Cannabis sativa
physiological function the FAD2 enzyme is responsible for the DELTA12 desaturation of oleic acid (18:1DELTA9) to linoleic acid (18:2DELTA9,12) Cannabis sativa