Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum cells | Synechocystis sp. |
gene desA, recombinant expression of the desA-licBM3 hybrid in transgenic Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum plants | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction, phenotypes, overview | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE | Synechocystis sp. |
66000 | - |
x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE | Synechocystis sp. |
66000 | - |
x * 66000, DesA As hybrid with lichenase LicBM3, SDS-PAGE | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Synechocystis sp. | - |
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
gene desA | - |
Synechocystis sp. | P20388 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Synechocystis sp. | 1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE | Synechocystis sp. |
? | x * 66000, DesA As hybrid with lichenase LicBM3, SDS-PAGE | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
acyl-lipid DELTA12-desaturase | - |
Synechocystis sp. |
DesA | - |
Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
additional information | expression profile of desA gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic, Escherichia coli and eukaryotic, Solanum tuberosum cells, overview | Synechocystis sp. |