Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Arthrospira platensis |
Protein Variants | Comment | Organism |
---|---|---|
D138N | inactive | Arthrospira platensis |
E140Q | inactive | Arthrospira platensis |
G136H | inactive | Arthrospira platensis |
H124R | inactive | Arthrospira platensis |
H128R | inactive | Arthrospira platensis |
H129R | inactive | Arthrospira platensis |
H305R | the mutant shows 17% of wild type activity | Arthrospira platensis |
H306R | inactive | Arthrospira platensis |
H313R | inactive | Arthrospira platensis |
H315N | inactive | Arthrospira platensis |
H89R | inactive | Arthrospira platensis |
H93R | the mutant shows 11% of wild type activity | Arthrospira platensis |
R123N | the mutant shows 91% of wild type activity | Arthrospira platensis |
W294G | inactive | Arthrospira platensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
linoleic acid | wild type enzyme, at pH 7.5 and 25°C | Arthrospira platensis | |
0.5 | - |
linoleic acid | mutant enzyme R123N, at pH 7.5 and 25°C | Arthrospira platensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Arthrospira platensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arthrospira platensis | - |
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrospira platensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arthrospira platensis | gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Arthrospira platensis |
Synonyms | Comment | Organism |
---|---|---|
DELTA6-acyl-lipid desaturase | - |
Arthrospira platensis |
desD | - |
Arthrospira platensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Arthrospira platensis |