Cloned (Comment) | Organism |
---|---|
gene CrDELTA4FAD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GFP-tagged enzyme in Chlamydomonas reinhardtii chloroplasts, recombinant expression of the His-tagged cytochrome b5 domain (resdues 48-147) in Escherichia coli strain BL21(DE3) | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | plastid envelope | Chlamydomonas reinhardtii | 9507 | - |
endoplasmic reticulum | - |
Chlamydomonas reinhardtii | 5783 | - |
membrane | membrane-bound enzyme, showing all characteristic features of a membrane-bound desaturase, including three histidine boxes and a transit peptide for chloroplast targeting | Chlamydomonas reinhardtii | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | I2CYZ4 | gene CrDELTA4FAD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged cytochrome b5 domain (residues 48-147) from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant His-tagged cytochrome b5 domain of the enzyme is active in vitro | Chlamydomonas reinhardtii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CrDELTA4FAD | - |
Chlamydomonas reinhardtii |
MGDG-specific DELTA4-desaturase | - |
Chlamydomonas reinhardtii |
monogalactosyldiacylglycerol-specific DELTA4-desaturase | - |
Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | cytochrome b5 is the primary electron donor for endoplasmic reticulum desaturases. The soluble cytochrome b5 domain corresponds to amino acids 48 to 147 | Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme knockdown lines show decreases in the 16:4 and 18:3-9,12,15 acyl groups in lipids, fatty acid profile, overview | Chlamydomonas reinhardtii |
additional information | overproduction of the enzyme in Chlamydomonas not only increases levels of 16:4 acyl groups in cell extracts but specifically increases the total amount of monogalactosyldiacylglycerol, fatty acid profile, overview | Chlamydomonas reinhardtii |
physiological function | the enzyme is involved in synthesis of the plastid lipid monogalactosyldiacylglycerol (MGDG) in the green alga Chlamydomonas reinhardtii occuring preferentially as a molecular species containing alpha-linolenic acid 18:3-9,12,15 in the sn-1 position and (4Z,7Z,10Z,13Z)-hexadeca-4,7,10,13-tetraenoic acid (16:4) in the sn-2 position of the glycerol backbone, fatty acid profile, overview | Chlamydomonas reinhardtii |