Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
A297L | the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor | Rattus norvegicus |
D299N | inactive | Rattus norvegicus |
D361N | the mutant shows increased Vmax compared to the wild type enzyme | Rattus norvegicus |
E362Q | the mutant shows reduced Vmax compared to the wild type enzyme | Rattus norvegicus |
E362R/E365R | the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor | Rattus norvegicus |
E365Q | the mutant shows reduced Vmax compared to the wild type enzyme | Rattus norvegicus |
K170E/L480A | inactive | Rattus norvegicus |
K366L | the mutant shows reduced Vmax compared to the wild type enzyme | Rattus norvegicus |
R298L | inactive | Rattus norvegicus |
R37E/R38E | the Km value for tetrahydrobiopterin measured for the mutant is approximately half that of the wild type enzyme | Rattus norvegicus |
S368A | the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dopamine | 1.8% residual activity at 0.01 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | - |
L-dopa + 4a-hydroxytetrahydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P04177 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-dopa + 4a-hydroxytetrahydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 70000, calculated from amino acid sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
tyrosine hydroxylase | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000595 | - |
pH and temperature not specified in the publication | Rattus norvegicus | dopamine |