Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild type and mutant S72A/V73I, the DELTA72 mutant, which lacks the first 72 amino acids, is not expressed in Escherichia coli at a detectable amount, suggesting that the truncated mutant cannot fold properly within the bacterial cell | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | modelling of CYP106A2 and site-directed mutagenesis of the protein to check the accuracy of the computer-derived model of CYP106A2 | Priestia megaterium |
S72A/V73I | mutant does not show a better stability in the crystallization process than the wild-type protein | Priestia megaterium |
General Stability | Organism |
---|---|
by adding the ligands imidazole and metyrapone, it is not possible to prevent CYP106A2 from degradation in the crystallization process | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
x * 47500 | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | Q06069 | ATCC 13368 | - |
Storage Stability | Organism |
---|---|
4°C, 4 weeks, degradation during storage | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the specificity of hydroxylation in beta-position can be altered by the choice of the electron transfer system. Replacing the natural electron transfer partners by peroxides, the ratio of 15alpha-/15beta-hydroxylation of progesterone is increased 1.3fold | Priestia megaterium | ? | - |
? | |
progesterone + reduced megaredoxin + O2 | - |
Priestia megaterium | 15beta-hydroxyprogesterone + oxidized megaredoxin + H2O | - |
? | |
testosterone + reduced adrenodoxin + O2 | - |
Priestia megaterium | 15beta-hydroxytestosterone + oxidized adrenodoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47500 | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
15beta-hydroxylase | - |
Priestia megaterium |
CYP106A2 | - |
Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Priestia megaterium |