Literature summary for 1.14.15.25 extracted from

Eaton, R.W.
p-Cymene catabolic pathway in Pseudomonas putida F1 cloning and characterization of DNA encoding conversion of p-cymene to p-cumate (1997), J. Bacteriol., 179, 3171-3180 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	O33456 and O33457	O33456 i.e. hydroxylase subunit CymAa, O33457 i.e. reductase subunit CymAb, cf. EC 1.8.1.3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme can attack benzylic and toluic alcohols as well as toluene and xylenes, probably with the formation of gem-diol intermediates which spontaneously dehydrate to yield the corresponding aldehydes	Pseudomonas putida	?	-	?
p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	-	Pseudomonas putida	4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 43117, hydrolase subunit CymAa, x * 38295, reductase subunit CymAb, calculated	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	reductase subunit CymAb	Pseudomonas putida
[2Fe-2S]-center	reductase subunit CymAb	Pseudomonas putida

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Release 2023.1 (January 2023)