Cloned (Comment) | Organism |
---|---|
mutant C334A transformed into chemically competent Escherichia coli NCM533 cells | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
C334A | is identical spectroscopically and enzymatically to wild-type but does not dimerize in solution at high concentrations | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | local protein backbone dynamics of CYP101 depend upon the oxidation and ligation state of the heme iron | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + O2 + reduced putidaredoxin | the reduced enzyme exhibits lower-amplitude motions of secondary structural features than the oxidized enzyme on all of the time scales accessible, and these differences are more pronounced in regions of the enzyme involved in substrate access to the active site (B' helix and beta3 and beta5 sheets) and binding of putidaredoxin (C and L helices), the iron-sulfur protein that acts as the effector and reductant of CYP101 in vivo | Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
camphor hydroxylase cytochrome P450cam | - |
Pseudomonas putida |
CYP101 | - |
Pseudomonas putida |
P450cam | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
putidaredoxin | - |
Pseudomonas putida |