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Literature summary for 1.14.14.94 extracted from

  • Kikuta, Y.; Kusunose, E.; Sumimoto, H.; Mizukami, Y.; Takeshige, K.; Sakaki, T.; Yabusaki, Y.; Kusunose, M.
    Purification and characterization of recombinant human neutrophil leukotriene B4 omega-hydroxylase (cytochrome P450 4F3) (1998), Arch. Biochem. Biophys., 355, 201-205.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in yeast cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CO recombinant protein, expressed in yeast Homo sapiens
Metyrapone recombinant protein, expressed in yeast, 35% inhibition at 2 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
leukotriene B4 recombinant protein CYP4F3, expressed in yeast Homo sapiens
0.06
-
leukotriene B4 recombinant protein CYP4F2, expressed in yeast Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
recombinant CYP4F3, expressed in yeast, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens inactivation of leukotriene B4, a mediator of inflammation (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein CYP4F3, expressed in yeast Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
30
-
recombinant protein, expressed in yeast Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 inactivation of leukotriene B4, a mediator of inflammation Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate Homo sapiens 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information recombinant protein CYP4F3, wide substrate specificity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
CYP4F3
-
Homo sapiens
cytochrome P450 4F3
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
recombinant protein, expressed in yeast Homo sapiens