Literature summary for 1.14.14.90 extracted from

Liu, C.J.; Huhman, D.; Sumner, L.W.; Dixon, R.A.
Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula (2003), Plant J., 36, 471-484.

Search for more literature for 1.14.14.90

Cloned(Commentary)

Cloned (Comment)	Organism
expression in yeast strain WAT11	Medicago truncatula

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	Biochanin A	pH 8.0	Medicago truncatula
0.067	-	formononetin	pH 8.0	Medicago truncatula

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Medicago truncatula	5783	-

Organism

Organism	UniProt	Comment	Textmining
Medicago truncatula	Q6WNR0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Medicago truncatula	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2	4.7% of the activity with biochanin A	Medicago truncatula	2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
biochanin A + [reduced NADPH-hemoprotein reductase] + O2	-	Medicago truncatula	2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
daidzein + [reduced NADPH-hemoprotein reductase] + O2	18.6% of the activity with biochanin A	Medicago truncatula	2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O	+ low concentrations of 3'-hydroxydaidzein	?
formononetin + [reduced NADPH-hemoprotein reductase] + O2	37.2% of the activity with biochanin A	Medicago truncatula	2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
genistein + [reduced NADPH-hemoprotein reductase] + O2	10.2% of the activity with biochanin A	Medicago truncatula	2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O	+ low concentrations of 3'-hydroxygenistaien	?
additional information	no activity with isoformononetin, prunetin, 6,7,4'-trihydroxyisoflavone, 3',4',7-trihydroxyisoflavone	Medicago truncatula	?	-	?

Synonyms

Synonyms	Comment	Organism
I2'H	-	Medicago truncatula
MtCYP81E7	-	Medicago truncatula

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.015	-	formononetin	pH 8.0	Medicago truncatula
0.033	-	Biochanin A	pH 8.0	Medicago truncatula

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Medicago truncatula

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Medicago truncatula

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Release 2023.1 (January 2023)