KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
formononetin | pH 8.0, 2 mM NADPH | Medicago truncatula | |
0.11 | - |
Biochanin A | pH 8.0, 2 mM NADPH | Medicago truncatula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Medicago truncatula | 5789 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | low mRNA levels | Medicago truncatula | - |
root | MRNA is found after arbuscular mycorrhizal colonization | Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2 | 39% of activity with biochanin A | Medicago truncatula | 2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Medicago truncatula | ? | - |
? | |
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | 50% of activity with biochanin A | Medicago truncatula | calycosin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
genistein + NADPH + O2 | 19% of activity with biochanin A | Medicago truncatula | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtCYP81E9 | - |
Medicago truncatula |
Mtl3'H | - |
Medicago truncatula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
formononetin | pH 8.0, 2 mM NADPH | Medicago truncatula | |
0.1 | - |
Biochanin A | pH 8.0, 2 mM NADPH | Medicago truncatula |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Medicago truncatula |