KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
(E)-phenylacetaldehyde oxime | pH 7.6, 30°C | Prunus mume | |
0.007 | - |
4-hydroxyphenylacetaldoxime | pH 7.6, 30°C | Prunus mume | |
0.0082 | - |
indole-3-acetaldoxime | pH 7.6, 30°C | Prunus mume | |
0.0185 | - |
phenylacetonitrile | pH 7.6, 30°C | Prunus mume | |
0.195 | - |
4-Hydroxyphenylacetonitrile | pH 7.6, 30°C | Prunus mume | |
0.751 | - |
indole-3-acetonitrile | pH 7.6, 30°C | Prunus mume |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-phenylacetaldehyde oxime | Prunus mume | - |
(Z)-phenylacetaldehyde oxime | - |
? | |
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | Prunus mume | - |
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
(Z)-phenylacetaldehyde oxime | Prunus mume | - |
phenylacetonitrile + H2O | - |
? | |
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | Prunus mume | - |
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus mume | A0A068Q609 | var. Sieb. et Zucc. | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Prunus mume | - |
seedling | - |
Prunus mume | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-phenylacetaldehyde oxime | - |
Prunus mume | (Z)-phenylacetaldehyde oxime | - |
? | |
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | - |
Prunus mume | (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
(Z)-phenylacetaldehyde oxime | - |
Prunus mume | phenylacetonitrile + H2O | - |
? | |
4-hydroxyphenylacetaldoxime + [reduced NADPH-hemoprotein reductase] + O2 | - |
Prunus mume | hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Prunus mume | hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
indole-3-acetaldoxime + [reduced NADPH-hemoprotein reductase] + O2 | reaction of EC 1.14.14.45 | Prunus mume | hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
indole-3-acetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | reaction of EC 1.14.14.45 | Prunus mume | hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
additional information | enzyme converts aromatic aldoximes and nitriles to the corresponding hydroxynitriles. No substrates: aliphatic substrates 2-methylpropanal oxime and 2-methylpropanenitrile | Prunus mume | ? | - |
? | |
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Prunus mume | (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP71AN24 | - |
Prunus mume |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
indole-3-acetaldoxime | pH 7.6, 30°C | Prunus mume | |
0.1 | - |
indole-3-acetonitrile | pH 7.6, 30°C | Prunus mume | |
0.4 | - |
phenylacetonitrile | pH 7.6, 30°C | Prunus mume | |
0.57 | - |
4-Hydroxyphenylacetonitrile | pH 7.6, 30°C | Prunus mume | |
0.62 | - |
4-hydroxyphenylacetaldoxime | pH 7.6, 30°C | Prunus mume | |
0.77 | - |
(E)-phenylacetaldehyde oxime | pH 7.6, 30°C | Prunus mume |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Prunus mume |