BRENDA - Enzyme Database
show all sequences of 1.14.14.30

Cloning, analysis, and overexpression of the gene encoding isobutylamine N-hydroxylase from the valanimycin producer, Streptomyces viridifaciens

Parry, R.J.; Li, W.; Cooper, H.N.; J. Bacteriol. 179, 409-416 (1997)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Streptomyces viridifaciens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Streptomyces viridifaciens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Streptomyces viridifaciens
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces viridifaciens
-
-
-
Streptomyces viridifaciens MG456-hF10
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
51.1
-
pH 7.5, 30°C
Streptomyces viridifaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-methylpropanamine + FMNH2 + O2
-
738507
Streptomyces viridifaciens
N-hydroxy-2-methylpropan-1-amine + FMN + H2O
-
-
-
?
2-methylpropanamine + FMNH2 + O2
-
738507
Streptomyces viridifaciens MG456-hF10
N-hydroxy-2-methylpropan-1-amine + FMN + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Streptomyces viridifaciens
Synonyms
Synonyms
Commentary
Organism
IBAH
-
Streptomyces viridifaciens
isobutylamine N-hydroxylase
-
Streptomyces viridifaciens
vlmH
-
Streptomyces viridifaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Streptomyces viridifaciens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Streptomyces viridifaciens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Streptomyces viridifaciens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
51.1
-
pH 7.5, 30°C
Streptomyces viridifaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-methylpropanamine + FMNH2 + O2
-
738507
Streptomyces viridifaciens
N-hydroxy-2-methylpropan-1-amine + FMN + H2O
-
-
-
?
2-methylpropanamine + FMNH2 + O2
-
738507
Streptomyces viridifaciens MG456-hF10
N-hydroxy-2-methylpropan-1-amine + FMN + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Streptomyces viridifaciens
Other publictions for EC 1.14.14.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
392301
Parry
An NADPH:FAD oxidoreductase fr ...
Streptomyces viridifaciens
J. Biol. Chem.
272
23303-23311
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737549
Parry
Purification and characterizat ...
Streptomyces viridifaciens, Streptomyces viridifaciens MG456-hF10
Arch. Biochem. Biophys.
339
47-54
1997
-
-
-
-
-
-
-
-
-
-
4
6
-
7
-
-
1
-
-
-
-
-
12
2
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
6
-
-
-
1
-
-
-
-
12
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
738507
Parry
Cloning, analysis, and overexp ...
Streptomyces viridifaciens, Streptomyces viridifaciens MG456-hF10
J. Bacteriol.
179
409-416
1997
-
-
1
-
-
-
-
-
1
-
1
-
-
9
-
-
-
-
-
-
1
-
2
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-