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Literature summary for 1.14.14.183 extracted from
- Molecular cloning and characterization of a cytochrome P450 taxoid 2alpha-hydroxylase involved in Taxol biosynthesis (2004), Arch. Biochem. Biophys., 427, 48-57 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in yeast | Taxus cuspidata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0105 | - |
taxusin | pH 7.8, 31°C | Taxus cuspidata |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7beta-hydroxytaxusin + [reduced NADPH-hemoprotein reductase] + O2 | Taxus cuspidata | - |
2alpha,7beta-dihydroxytaxusin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| taxusin + [reduced NADPH-hemoprotein reductase] + O2 | Taxus cuspidata | - |
2alpha-hydroxytaxusin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Taxus cuspidata | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7beta-hydroxytaxusin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Taxus cuspidata | 2alpha,7beta-dihydroxytaxusin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| taxusin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Taxus cuspidata | 2alpha-hydroxytaxusin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE, recombinant protein | Taxus cuspidata |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
taxusin | pH 7.8, 31°C | Taxus cuspidata | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Taxus cuspidata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Taxus cuspidata |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | taxusin is hydroxylated by both taxoid 7beta-hydroxylase and taxoid 2alpha-hydroxylase, and both enzymes are capable of the reciprocal conversion of their respective pentaol tetraacetate products to the hexaol tetraacetate | Taxus cuspidata |
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