Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | saturating amounts increase activity | Taxus cuspidata | |
FAD | saturating amounts increase activity | Taxus brevifolia | |
FMN | saturating amounts increase activity | Taxus cuspidata | |
FMN | saturating amounts increase activity | Taxus brevifolia |
Application | Comment | Organism |
---|---|---|
medicine | enzyme may be useful in the biological production of the potent antimitotic drug taxol which shows activity against a range of cancers | Taxus cuspidata |
medicine | enzyme may be useful in the biological production of the potent antimitotic drug taxol which shows activity against a range of cancers | Taxus brevifolia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clotrimazole | 0.003 mM, complete inhibition | Taxus brevifolia | |
clotrimazole | 0.003 mM, complete inhibition | Taxus cuspidata | |
CO | inhibits taxadiene hydroxylation in the dark, inhihibition is partially reversed by blue light at 450 nm | Taxus brevifolia | |
CO | inhibits taxadiene hydroxylation in the dark, inhihibition is partially reversed by blue light at 450 nm | Taxus cuspidata | |
cytochrome c | 0.4 mM, 50% inhibition | Taxus brevifolia | |
cytochrome c | 0.4 mM, 50% inhibition | Taxus cuspidata | |
miconazole | 0.003 mM, complete inhibition | Taxus brevifolia | |
miconazole | 0.003 mM, complete inhibition | Taxus cuspidata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
taxa-4,11-diene | approx. value | Taxus cuspidata | |
0.006 | - |
taxa-4,11-diene | approx. value | Taxus brevifolia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | 70% activity resides in light membrane fraction, approx. 25% in dense membrane fraction | Taxus cuspidata | - |
- |
microsome | 70% activity resides in light membrane fraction, approx. 25% in dense membrane fraction | Taxus brevifolia | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 | Taxus cuspidata | reaction is completely dependent on NADPH, enzyme catalyzes the first oxygenating step in taxol biosynthesis pathway | taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 | Taxus brevifolia | reaction is completely dependent on NADPH, enzyme catalyzes the first oxygenating step in taxol biosynthesis pathway | taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus brevifolia | - |
- |
- |
Taxus cuspidata | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 = taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | requires P450, reaction includes rearrangement of the 4(5)-double bound to a 4(20)-double bond, possibly through allylic oxidation, proposed mechanism | Taxus cuspidata | |
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 = taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | requires P450, reaction includes rearrangement of the 4(5)-double bound to a 4(20)-double bond, possibly through allylic oxidation, proposed mechanism | Taxus brevifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Taxus cuspidata | - |
stem | - |
Taxus brevifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 | reaction is completely dependent on NADPH | Taxus cuspidata | taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 | reaction is completely dependent on NADPH | Taxus brevifolia | taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 | reaction is completely dependent on NADPH, enzyme catalyzes the first oxygenating step in taxol biosynthesis pathway | Taxus cuspidata | taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 | reaction is completely dependent on NADPH, enzyme catalyzes the first oxygenating step in taxol biosynthesis pathway | Taxus brevifolia | taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
activity in extracts, approx. 50% of maximal activity at pH 6.2 and pH 8.2 | Taxus cuspidata |
7.2 | - |
activity in extracts, approx. 50% of maximal activity at pH 6.2 and pH 8.2 | Taxus brevifolia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | absolutely required | Taxus cuspidata | |
NADPH | absolutely required | Taxus brevifolia |