Cloned (Comment) | Organism |
---|---|
expression in yeast | Inula hupehensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 | Inula hupehensis | the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone | 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Inula hupehensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Inula hupehensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots | Inula hupehensis | - |
leaf | the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots | Inula hupehensis | - |
root | the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots | Inula hupehensis | - |
stem | the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots | Inula hupehensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 | the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone | Inula hupehensis | 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 | the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide | Inula hupehensis | 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 | overall reaction. The product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide | Inula hupehensis | 8-epi-inunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP71BL6 | - |
Inula hupehensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Inula hupehensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Inula hupehensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | heme-thiolate protein | Inula hupehensis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone | Inula hupehensis |