BRENDA - Enzyme Database
show all sequences of 1.14.14.170

Discovery of a non-stereoselective cytochrome P450 catalyzing either 8alpha- or 8beta-hydroxylation of germacrene A acid from the Chinese medicinal plant, Inula hupehensis

Gou, J.; Hao, F.; Huang, C.; Kwon, M.; Chen, F.; Li, C.; Liu, C.; Ro, D.K.; Tang, H.; Zhang, Y.; Plant J. 93, 92-106 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in yeast
Inula hupehensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Inula hupehensis
the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Inula hupehensis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Inula hupehensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
leaf
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
root
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
stem
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone
751862
Inula hupehensis
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide
751862
Inula hupehensis
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
overall reaction. The product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide
751862
Inula hupehensis
8-epi-inunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Inula hupehensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Inula hupehensis
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
heme-thiolate protein
Inula hupehensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in yeast
Inula hupehensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
heme-thiolate protein
Inula hupehensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Inula hupehensis
the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Inula hupehensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
leaf
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
root
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
stem
the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots
Inula hupehensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone
751862
Inula hupehensis
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide
751862
Inula hupehensis
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
overall reaction. The product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide
751862
Inula hupehensis
8-epi-inunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Inula hupehensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Inula hupehensis
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone
Inula hupehensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone
Inula hupehensis
Other publictions for EC 1.14.14.170
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751862
Gou
Discovery of a non-stereosele ...
Inula hupehensis
Plant J.
93
92-106
2018
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
4
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
4
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-