Crystallization (Comment) | Organism |
---|---|
in omplex with 17alpha-hydroxyprogesterone, to 3.3 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G292C | 0.32% of wild-type efficiency with substrate progesterone | Homo sapiens |
G292S | 0.0005% of wild-type efficiency with substrate progesterone | Homo sapiens |
G65E | 0.082% of wild-type efficiency with substrate progesterone | Homo sapiens |
I172N | 0.006% of wild-type efficiency with substrate progesterone | Homo sapiens |
L108R | 0.0003% of wild-type efficiency with substrate progesterone | Homo sapiens |
P31L | 2.4% of wild-type efficiency with substrate progesterone | Homo sapiens |
P31Q | 0.0001% of wild-type efficiency with substrate progesterone | Homo sapiens |
R357W | 0.01% of wild-type efficiency with substrate progesterone | Homo sapiens |
R409C | 0.4% of wild-type efficiency with substrate progesterone | Homo sapiens |
T296N | 0.05% of wild-type efficiency with substrate progesterone | Homo sapiens |
V282L | 2% of wild-type efficiency with substrate progesterone | Homo sapiens |
W303R | 0.0001% of wild-type efficiency with substrate progesterone | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00039 | - |
progesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00044 | - |
17alpha-hydroxyprogesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00046 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0011 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0012 | - |
progesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0012 | - |
progesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0014 | - |
17alpha-hydroxyprogesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0015 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0017 | - |
17alpha-hydroxyprogesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0017 | - |
17alpha-hydroxyprogesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0018 | - |
progesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0024 | - |
17alpha-hydroxyprogesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0027 | - |
progesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0036 | - |
17alpha-hydroxyprogesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0038 | - |
progesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0046 | - |
17alpha-hydroxyprogesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0047 | - |
progesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0049 | - |
17alpha-hydroxyprogesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0055 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0073 | - |
17alpha-hydroxyprogesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0096 | - |
progesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.013 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.022 | - |
progesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.022 | - |
17alpha-hydroxyprogesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.11 | - |
progesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08686 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ + O2 | - |
Homo sapiens | 17,21-dihydroxyprogesterone + NADP+ + H2O | - |
? | |
additional information | C-H bond breaking is a rate-limiting step over a 10000fold range of catalytic efficiency | Homo sapiens | ? | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Homo sapiens | 11-deoxycorticosterone + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome p450 21A2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00003 | - |
17alpha-hydroxyprogesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.000033 | - |
17alpha-hydroxyprogesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00005 | - |
17alpha-hydroxyprogesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0001 | - |
progesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0001 | - |
progesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00013 | - |
progesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00022 | - |
17alpha-hydroxyprogesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00027 | - |
progesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0005 | - |
17alpha-hydroxyprogesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00083 | - |
progesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0015 | - |
progesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.003 | - |
17alpha-hydroxyprogesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.008 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.02 | - |
progesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.028 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.03 | - |
17alpha-hydroxyprogesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.032 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.033 | - |
17alpha-hydroxyprogesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.06 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.063 | - |
17alpha-hydroxyprogesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.2 | - |
progesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.38 | - |
progesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
1.033 | - |
progesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
1.4 | - |
17alpha-hydroxyprogesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.8 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
4 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
progesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.012 | - |
progesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.013 | - |
progesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.016 | - |
17alpha-hydroxyprogesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.02 | - |
17alpha-hydroxyprogesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.02 | - |
17alpha-hydroxyprogesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.03 | - |
progesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.03 | - |
17alpha-hydroxyprogesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.07 | - |
progesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.12 | - |
17alpha-hydroxyprogesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.62 | - |
17alpha-hydroxyprogesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.82 | - |
progesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
6.7 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
11.2 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
13.3 | - |
17alpha-hydroxyprogesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
43.3 | - |
progesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
50 | - |
progesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
61.7 | - |
17alpha-hydroxyprogesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
66.7 | - |
17alpha-hydroxyprogesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
100 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
267 | - |
progesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
317 | - |
progesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
817 | - |
17alpha-hydroxyprogesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2667 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
13333 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |