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Literature summary for 1.14.14.103 extracted from
- A cytochrome P-450 monooxygenase catalyzes the first step in the conversion of tabersonine to vindoline in Catharanthus roseus (1995), Plant Physiol., 109, 131-139.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| clotrimazole | 50% inhibition at 0.05 mM | Catharanthus roseus |  |
| CO | concentration dependent competitive inhibition, 77% inhibition at 90% | Catharanthus roseus | |
| cytochrome c | 50% inhibition at 0.001 mM | Catharanthus roseus | |
| miconazole | 50% inhibition at 0.3 mM | Catharanthus roseus | |
| tabersonine | inhibition above 0.03 mM | Catharanthus roseus | |
| tricliphane | 50% inhibition at 0.5 mM | Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
tabersonine | - |
Catharanthus roseus | |
| 0.014 | - |
NADPH | - |
Catharanthus roseus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Catharanthus roseus | 5783 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tabersonine + NADPH + H+ + O2 | Catharanthus roseus | first step in vindoline boxynthesis | 16-hydroxytabersonine + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Catharanthus roseus | - |
| leaf | - |
Catharanthus roseus | - |
| root | - |
Catharanthus roseus | - |
| seedling | - |
Catharanthus roseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tabersonine + NADPH + H+ + O2 | NADH is less active as substrate | Catharanthus roseus | 16-hydroxytabersonine + NADP+ + H2O | - |
? | |
| tabersonine + NADPH + H+ + O2 | first step in vindoline boxynthesis | Catharanthus roseus | 16-hydroxytabersonine + NADP+ + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
phosphate buffer | Catharanthus roseus |
| 8 | - |
Tris buffer | Catharanthus roseus |
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Release 2023.1 (January 2023)
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