Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozymes FMO1 and FMO3 as N-terminally maltose-binding-protein fusion and C-terminally His6-tagged proteins in Escherichia coli strain JM109 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H360P | site-directed mutagenesis of isozyme FMO1, the mutant shows altered thermal stability and highly increased activity with mercaptoimidazole and chlorpromazine compared to the wild-type FMO1 | Homo sapiens |
L360A | site-directed mutagenesis of isozyme FMO3, the mutant shows altered thermal stability and reduced activity with mercaptoimidazole, chlorpromazine, and 10-[(N,N-dimethylaminopentyl)-2-(trifluoromethyl)]phenothiazine compared to the wild-type FMO3 | Homo sapiens |
L360H | site-directed mutagenesis of isozyme FMO3, the mutant shows altered thermal stability and reduced activity with mercaptoimidazole, chlorpromazine, and 10-[(N,N-dimethylaminopentyl)-2-(trifluoromethyl)]phenothiazine compared to the wild-type FMO3 | Homo sapiens |
L360P | site-directed mutagenesis of isozyme FMO3, the mutant shows altered thermal stability and increased activity with mercaptoimidazole, chlorpromazine, and 10-[(N,N-dimethylaminopentyl)-2-(trifluoromethyl)]phenothiazine compared to the wild-type FMO3 | Homo sapiens |
L360Q | site-directed mutagenesis of isozyme FMO3, the mutant shows altered thermal stability and reduced activity with mercaptoimidazole, chlorpromazine, and 10-[(N,N-dimethylaminopentyl)-2-(trifluoromethyl)]phenothiazine compared to the wild-type FMO3 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.02 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant mutant H360P His6-tagged MBT-fusion-FMO1 | Homo sapiens | |
0.022 | - |
chlorpromazine | pH 8.4, 37°C, recombinant mutant H360P His6-tagged MBT-fusion-FMO1 | Homo sapiens | |
0.022 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant mutant L360Q His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.026 | - |
chlorpromazine | pH 8.4, 37°C, recombinant mutant L360P His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.028 | - |
chlorpromazine | pH 8.4, 37°C, recombinant mutant L360H His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.03 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO1 | Homo sapiens | |
0.034 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant mutant L360P His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.038 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant mutant L360H His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.046 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant mutant L360A His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.058 | - |
chlorpromazine | pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO1 | Homo sapiens | |
0.061 | - |
chlorpromazine | pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.076 | - |
chlorpromazine | pH 8.4, 37°C, recombinant mutant L360Q His6-tagged MBT-fusion-FMO3 | Homo sapiens | |
0.08 | - |
chlorpromazine | pH 8.4, 37°C, recombinant mutant L360A His6-tagged MBT-fusion-FMO3 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes FMO1 and FMO3 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity of recombinant wild-type and mutant His6-tagged MBT-fusion-FMO3s, overview | Homo sapiens |
0.0046 | 0.0073 | recombinant wild-type and mutant His6-tagged MBT-fusion-FMO3s | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-[(N,N-dimethylaminopentyl)-2-(trifluoromethyl)]phenothiazine + NADPH + O2 | i.e. 5-DPT or diethylenetriaminepentaacetic acid | Homo sapiens | ? | - |
? | |
chlorpromazine + NADPH + O2 | - |
Homo sapiens | chlorpromazine N-oxide + NADP+ + H2O | - |
? | |
mercaptoimidazole + NADPH + O2 | - |
Homo sapiens | mercaptoimidazole S-oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling of the FMO3 structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FMO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
3 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
in absence of NADPH, residue 360 is important for thermal stability, half-lives of wild-type and mutant isozymes FMO1 and FMO3, overview | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |