Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D597N/M336V | mutant of nNOS, the Ki values for the nNOS double mutant increase for all inhibitors but the mutant still binds these inhibitors better than eNOS | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4S)-N-(4-amino-5-[aminoethyl]aminopentyl)-N''-nitroguanidine | - |
Homo sapiens | |
3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-ol | - |
Homo sapiens | |
L-Nomega-nitroarginine-(4R)-amino-L-proline amide | - |
Homo sapiens | |
L-Nomega-nitroarginine-2,4-L-diaminobutyramide | - |
Homo sapiens | |
N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamine | - |
Homo sapiens | |
N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamine | - |
Homo sapiens | |
N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
L-arginine | mutant nNOS D597N/M336V | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29474 | - |
- |
Homo sapiens | P29475 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose column chromatography, 2',5'-ADP Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Homo sapiens | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endothelial nitric oxide synthase | - |
Homo sapiens |
eNOS | - |
Homo sapiens |
neuronal nitric oxide synthase | - |
Homo sapiens |
nNOS | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamine | wild type nNOS | Homo sapiens | |
0.0001 | - |
L-Nomega-nitroarginine-(4R)-amino-L-proline amide | wild type nNOS | Homo sapiens | |
0.00015 | - |
(4S)-N-(4-amino-5-[aminoethyl]aminopentyl)-N''-nitroguanidine | wild type nNOS | Homo sapiens | |
0.00025 | - |
N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamine | wild type nNOS | Homo sapiens | |
0.0003 | - |
L-Nomega-nitroarginine-2,4-L-diaminobutyramide | wild type nNOS | Homo sapiens | |
0.000388 | - |
N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamine | wild type nNOS | Homo sapiens | |
0.0012 | - |
N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamine | mutant nNOS D597N/M336V | Homo sapiens | |
0.0061 | - |
N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamine | mutant nNOS D597N/M336V | Homo sapiens | |
0.0094 | - |
3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-ol | wild type nNOS | Homo sapiens | |
0.0367 | - |
N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamine | mutant nNOS D597N/M336V | Homo sapiens | |
0.0492 | - |
3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-ol | mutant nNOS D597N/M336V | Homo sapiens | |
0.08 | - |
(4S)-N-(4-amino-5-[aminoethyl]aminopentyl)-N''-nitroguanidine | wild type eNOS | Homo sapiens | |
0.0852 | - |
N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamine | wild type eNOS | Homo sapiens | |
0.0952 | - |
N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamine | wild type eNOS | Homo sapiens | |
0.107 | - |
L-Nomega-nitroarginine-2,4-L-diaminobutyramide | wild type eNOS | Homo sapiens | |
0.11 | - |
L-Nomega-nitroarginine-(4R)-amino-L-proline amide | wild type eNOS | Homo sapiens | |
0.3666 | - |
3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-ol | wild type eNOS | Homo sapiens | |
0.4167 | - |
N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamine | wild type eNOS | Homo sapiens |