BRENDA - Enzyme Database
show all sequences of 1.14.13.170

Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone

Seo, M.J.; Zhu, D.; Endo, S.; Ikeda, H.; Cane, D.E.; Biochemistry 50, 1739-1754 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces exfoliatus
expressed in Escherichia coli BL21(DE3) cells
Streptomyces arenae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66915
-
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces arenae
67480
-
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces exfoliatus
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces arenae
-
-
-
Streptomyces arenae TU46
-
-
-
Streptomyces exfoliatus
-
-
-
Streptomyces exfoliatus UC5319
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography
Streptomyces exfoliatus
Ni-NTA column chromatography
Streptomyces arenae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces exfoliatus
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces arenae
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces arenae TU46
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces exfoliatus UC5319
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces arenae TUE469
pentalenolactone D + NADP+ + H2O
-
-
-
ir
Subunits
Subunits
Commentary
Organism
?
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces arenae
?
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces exfoliatus
Synonyms
Synonyms
Commentary
Organism
Baeyer-Villiger monooxygenase
-
Streptomyces exfoliatus
Baeyer-Villiger monooxygenase
-
Streptomyces arenae
penE
-
Streptomyces exfoliatus
pntE
-
Streptomyces arenae
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
the enzyme contains one bound FAD molecule
Streptomyces exfoliatus
FAD
the enzyme contains one bound FAD molecule
Streptomyces arenae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces exfoliatus
expressed in Escherichia coli BL21(DE3) cells
Streptomyces arenae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
the enzyme contains one bound FAD molecule
Streptomyces exfoliatus
FAD
the enzyme contains one bound FAD molecule
Streptomyces arenae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66915
-
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces arenae
67480
-
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces exfoliatus
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Streptomyces exfoliatus
Ni-NTA column chromatography
Streptomyces arenae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces exfoliatus
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces arenae
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces arenae TU46
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces exfoliatus UC5319
pentalenolactone D + NADP+ + H2O
-
-
-
ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
718892
Streptomyces arenae TUE469
pentalenolactone D + NADP+ + H2O
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces arenae
?
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry
Streptomyces exfoliatus
Other publictions for EC 1.14.13.170
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718892
Seo
Genome mining in Streptomyces. ...
Streptomyces arenae, Streptomyces exfoliatus, Streptomyces arenae TU46, Streptomyces exfoliatus UC5319
Biochemistry
50
1739-1754
2011
-
-
2
-
-
-
-
-
-
-
2
-
-
7
-
-
2
-
-
-
-
-
5
2
4
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
5
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-