Cloned (Comment) | Organism |
---|---|
gene vppD cloned from a 97-kbp DNA fragment containing six nicotine degradation-related genes which is obtained by gap closing from the genome sequence of strain SJY1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Ochrobactrum sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | strong inhibition | Ochrobactrum sp. | |
Cu2+ | strong inhibition | Ochrobactrum sp. | |
Zn2+ | strong inhibition | Ochrobactrum sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
NADH | pH 8.0, 25°C, recombinant His-tagged enzyme | Ochrobactrum sp. | |
0.201 | - |
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate | pH 8.0, 25°C, recombinant His-tagged enzyme | Ochrobactrum sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | Ochrobactrum sp. | - |
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ochrobactrum sp. | A0A075XAG5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. FAD is partly lost during the purification | Ochrobactrum sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:nicotinic acid-grown cell | strain SJY uses nicotine as a sole source of carbon, nitrogen, and energy | Ochrobactrum sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Ochrobactrum sp. | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VppD | - |
Ochrobactrum sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Ochrobactrum sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.5 | - |
NADH | pH 8.0, 25°C, recombinant His-tagged enzyme | Ochrobactrum sp. | |
21.6 | - |
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate | pH 8.0, 25°C, recombinant His-tagged enzyme | Ochrobactrum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Ochrobactrum sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Ochrobactrum sp. | |
NADH | - |
Ochrobactrum sp. |
General Information | Comment | Organism |
---|---|---|
evolution | sequence alignment and phylogenetic analysis suggests that the VPP pathway, which evolved independently from nicotinic acid degradation, might have a closer relationship with the pyrrolidine pathway | Ochrobactrum sp. |
metabolism | strain SJY1 efficiently degrades nicotine via a variant of the pyridine and pyrrolidine pathways (the VPP pathway), highlighting bacterial metabolic diversity in relation to nicotine degradation, a 97-kbp DNA fragment containing six nicotine degradation-related genes is obtained by gap closing from the genome sequence of strain SJY1, gene vppD gene encodes an NADH-dependent flavin-containing monooxygenase, which catalyzes the hydroxylation of 6-hydroxy-3-succinoylpyridine to 2,5-dihydroxypyridine. Nicotine degradation pathway in strain SJY1, detailed overview | Ochrobactrum sp. |
additional information | the ativity of VppD is 10fold higher than the activity of the hydroxylase (HspB) from Pseudomonas putida strain S16 | Ochrobactrum sp. |