Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
in complex with flavin adenine dinucleotide at a 1.95 A resolution as well as with bound FAD and NADP+ at a 2.0 A resolution. Crystal forms reveal a conformational plasticity of several loop regions | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
0.03 | - |
2-n-hexylcyclopentanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
0.16 | - |
2-methylcyclohexanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
0.31 | - |
2-oxocyclopentyl ethyl acetate | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
0.6 | - |
2-n-hexylcyclopentanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
1.4 | - |
4-methylcyclohexanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61480 | - |
x * 62000, SDS-PAGE, x * 61480, calculated | Pseudomonas putida |
62000 | - |
x * 62000, SDS-PAGE, x * 61480, calculated | Pseudomonas putida |
118000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | H3JQW0 | - |
- |
Pseudomonas putida ATCC 17453 | H3JQW0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ethylcyclopentanone + O2 + NADPH + H+ | - |
Pseudomonas putida | 6-ethyltetrahydro-2H-pyran-2-one + NADP+ + H2O | both enantiomers of 2-ethyl cyclohexanone are oxidized at nearly the same rate although giving a low enantiomeric ratio | ? | |
2-ethylcyclopentanone + O2 + NADPH + H+ | - |
Pseudomonas putida ATCC 17453 | 6-ethyltetrahydro-2H-pyran-2-one + NADP+ + H2O | both enantiomers of 2-ethyl cyclohexanone are oxidized at nearly the same rate although giving a low enantiomeric ratio | ? | |
2-methylcyclohexanone + O2 + NADPH + H+ | - |
Pseudomonas putida | 7-methyloxepan-2-one + NADP+ + H2O | - |
? | |
2-methylcyclohexanone + O2 + NADPH + H+ | - |
Pseudomonas putida ATCC 17453 | 7-methyloxepan-2-one + NADP+ + H2O | - |
? | |
2-n-hexylcyclopentanone + O2 + NADPH + H+ | - |
Pseudomonas putida | 6-hexyltetrahydro-2H-pyran-2-one + NADP+ + H2O | - |
? | |
2-n-hexylcyclopentanone + O2 + NADPH + H+ | - |
Pseudomonas putida ATCC 17453 | 6-hexyltetrahydro-2H-pyran-2-one + NADP+ + H2O | - |
? | |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid + O2 + NADPH + H+ | - |
Pseudomonas putida ATCC 17453 | ? + NADP+ + H2O | - |
? | |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
2-oxocyclopentyl acetic acid + O2 + NADPH + H+ | - |
Pseudomonas putida | (6-oxotetrahydro-2H-pyran-2-yl)acetic acid + NADP+ + H2O | - |
? | |
2-oxocyclopentyl ethyl acetate + O2 + NADPH + H+ | - |
Pseudomonas putida | ethyl (6-oxotetrahydro-2H-pyran-2-yl)acetate + NADP+ + H2O | - |
? | |
4-methylcyclohexanone + O2 + NADPH + H+ | - |
Pseudomonas putida | 5-methyloxepan-2-one + NADP+ + H2O | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | products are a mixture of normal to abnormal lactone at a ratio of 3:1 | ? | |
additional information | reaction of different 4-substituted cyclohexanones with small to medium substituents at the C-4 position in nearly all cases give enantiopure lactones with an excellent yield | Pseudomonas putida | ? | - |
? | |
additional information | reaction of different 4-substituted cyclohexanones with small to medium substituents at the C-4 position in nearly all cases give enantiopure lactones with an excellent yield | Pseudomonas putida ATCC 17453 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x * 62000, SDS-PAGE, x * 61480, calculated | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
OTEMO | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable up to | Pseudomonas putida |
40 | - |
half-life 3.8 min | Pseudomonas putida |
45 | - |
half-life below 0.4 min | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2-oxocyclopentyl acetic acid | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
0.13 | - |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
0.9 | - |
2-methylcyclohexanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
1.8 | - |
2-n-hexylcyclopentanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
4.8 | - |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
5.6 | - |
4-methylcyclohexanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
6.9 | - |
2-oxocyclopentyl ethyl acetate | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
13.6 | - |
2-n-hexylcyclopentanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | Crystallization data. With the exception of the O2' atom of ribose and the C-8 and N-9 atoms of adenine, the FAD cofactor is completely buried and inaccessible to the solvent | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
2-n-hexylcyclopentanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
4 | - |
4-methylcyclohexanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
5.5 | - |
2-methylcyclohexanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
22 | - |
2-oxocyclopentyl ethyl acetate | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
270 | - |
2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
430 | - |
2-n-hexylcyclopentanone | pH 9.0, temperature not specified in the publication | Pseudomonas putida |