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Literature summary for 1.14.13.148 extracted from

  • Large, P.; Boulton, C.; Crabbe, M.
    The reduced nicotinamide-adenine dinucleotide phosphate- and oxygen-dependent N-oxygenation of trimethylamine by Pseudomonas aminovorans (1972), Biochem. J., 128, 137P-138P.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.148

Inhibitors

Inhibitors Comment Organism Structure
3-dimethylaminopropan-1-ol inhibitory at concentrations above about 1 mM Aminobacter aminovorans
3-dimethylaminopropan-2-ol inhibitory at concentrations above about 1 mM Aminobacter aminovorans
Ca2+
-
 Aminobacter aminovorans
Co2+
-
 Aminobacter aminovorans
diethylmethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
ethyldimethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
additional information the enzyme is not inhibited by CO, cyanide or SKF 525-A (2-diethylaminoethyl 2,2-diphenylvalerate hydrochloride), nor by chelating agents Aminobacter aminovorans
N,N-dimethyl-2-chloroethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
N,N-dimethylethanolamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
N,N-dimethylethylenediamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
N,N-dimethylpropylenediamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
triethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
Zn2+
-
 Aminobacter aminovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NADPH pH and temperature not specified in the publication Aminobacter aminovorans
2
-
 NADH pH and temperature not specified in the publication Aminobacter aminovorans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme shows no metal ion requirement Aminobacter aminovorans

Organism

Organism UniProt Comment Textmining
Aminobacter aminovorans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dimethylaminopropan-1-ol + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
3-dimethylaminopropan-2-ol + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
diethylmethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
ethyldimethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
N,N,N-trimethylamine + NADH + H+ + O2
-
 Aminobacter aminovorans N,N,N-trimethylamine N-oxide + NAD+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N-dimethyl-2-chloroethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
N,N-dimethylethanolamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
N,N-dimethylethylenediamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
N,N-dimethylpropylenediamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
triethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 the enzyme has a half-life in the crude extract of 20 min at 35°C Aminobacter aminovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme is stable at 0°C for 30min at any pH value in the range 4.0-9.0 Aminobacter aminovorans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Aminobacter aminovorans
NADPH dependent on, preferred electron donor over NADH Aminobacter aminovorans