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Literature summary for 1.14.13.148 extracted from
- The effect of arginine-428 mutation on modulation of activity of human liver flavin monooxygenase 3 (FMO3) by imipramine and chlorpromazine (1999), Exp. Toxicol. Pathol., 51, 271-276.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chlorpromazine | the activity of the native enzyme is increased at 0.001-0.2 mM of chlorpromazine. Greatest activation is 85% when methimazole and chlorpromazine concentrations are 2 mM and 0.1 mM, respectively | Homo sapiens |  |
| imipramine | the activity of the native enzyme is increased at 0.05-0.75 mM of imipramine | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T428R | methimazole activity of the mutant enzyme is stimulated (maximally 25% when the methimazole concentration is 2 mM) to the same extend of native enzyme up to an imipramine concentration of 3 mM. The activity of the mutant is inhibited at concentrations above 0.3 mM imipramine, 0.75 mM imipramine causes 93% inhibition of methimazole activity of the mutant enzyme. Chlorpromazine activates the mutant enzyme only at high substrate concentrations (0.1-2 mM) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0285 | - |
Methimazole | mutant enzyme, at pH and 37°C | Homo sapiens | |
| 0.03 | - |
Methimazole | native enzyme, at pH and 37°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methimazole + NADPH + H+ + O2 | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EC 1.14.13.8 | assigned to | Homo sapiens |
| flavin monooxygenase 3 | - |
Homo sapiens |
| flavin-containing monooxygenase | - |
Homo sapiens |
| FMO3 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| NADPH | required for activity | Homo sapiens | |
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Release 2023.1 (January 2023)
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