Crystallization (Comment) | Organism |
---|---|
crystal structures of the nonreduced, reduced, and substrate-bound binary complexes of terminal oxygenase CARDO-O from Janthinobacterium sp. J3 with its electron donor, ferredoxin CARDO-F from Pseudomonas resinovorans CA10 at 1.9, 1.8, and 2.0 A resolutions, respectively. The structures provide a structure-based interpretation of intercomponent electron transfer between two Rieske [2Fe-2S] clusters of ferredoxin and oxygenase in a Rieske nonheme iron oxygenase system. Three molecules of CARDO-F bind to the subunit boundary of one CARDO-O trimeric molecule, and specific binding created by electrostatic and hydrophobic interactions with conformational changes suitably aligns the two Rieske clusters for electron transfer. Additionally, conformational changes upon binding carbazole results in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site | Pseudomonas resinovorans |
crystal structures of the nonreduced, reduced, and substrate-bound binary complexes of terminal oxygenase CARDO-O from Janthinobacterium sp. J3 with its electron donor, ferredoxin CARDO-F from Pseudomonas resinovorans CA10 at 1.9, 1.8, and 2.0 A resolutions, respectively. The structures provide a structure-based interpretation of intercomponent electron transfer between two Rieske [2Fe-2S] clusters of ferredoxin and oxygenase in a Rieske nonheme iron oxygenase system. Three molecules of CARDO-F bind to the subunit boundary of one CARDO-O trimeric molecule, and specific binding created by electrostatic and hydrophobic interactions with conformational changes suitably aligns the two Rieske clusters for electron transfer. Additionally, conformational changes upon binding carbazole results in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site | Janthinobacterium sp. J3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | ferredoxin component contains two Rieske [2Fe-2S] clusters | Pseudomonas resinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Janthinobacterium sp. J3 | - |
- |
- |
Pseudomonas resinovorans | - |
- |
- |
Pseudomonas resinovorans CA10 | - |
- |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | ferredoxin component contains two Rieske [2Fe-2S] clusters | Pseudomonas resinovorans |