Literature summary for 1.14.11.35 extracted from

You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.; Jogl, G.
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis (2007), J. Biol. Chem., 282, 36552-36560.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Streptomyces avermitilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
complexes with the cofactors iron, alpha-ketoglutarate, and the nonreactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold and the cofactor-binding site for iron and alpha-keto-glutarate is similar to other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate	Streptomyces avermitilis

Crystallization (Comment)

Organism

complexes with the cofactors iron, alpha-ketoglutarate, and the nonreactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold and the cofactor-binding site for iron and alpha-keto-glutarate is similar to other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate

Streptomyces avermitilis

Protein Variants

Protein Variants	Comment	Organism
R117Q	complete loss of activity	Streptomyces avermitilis
R188Q	280fold decrease in activity	Streptomyces avermitilis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces avermitilis	Q82IZ1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no substrate: ent-1-deoxypentalenic acid	Streptomyces avermitilis	?	-	?

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Release 2023.1 (January 2023)