Cloned (Comment) | Organism |
---|---|
cloning of the alpha-I subunit and of a second mouse alpha-subunit isoform, termed the alpha-II subunit, expression of the alpha-II subunit together with human protein disulfide isomerase/beta subunit in insect cells by baculovirus vectors | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
poly(L-proline) | inhibitory, type I enzyme tetramer, MW: 7000 and 44000 | Homo sapiens | |
poly(L-proline) | inhibitor, recombinant type II enzyme tetramer, MW: 7000 and 44000 | Mus musculus | |
Pyridine 2,4-dicarboxylate | inhibitory, type I enzyme tetramer | Homo sapiens | |
Pyridine 2,4-dicarboxylate | inhibitory, recombinant type II enzyme tetramer | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2-oxoglutarate | recombinant type II enzyme tetramer | Mus musculus | |
0.018 | - |
(Pro-Pro-Gly)10 | type I enzyme tetramer | Homo sapiens | |
0.022 | - |
2-oxoglutarate | type I enzyme tetramer | Homo sapiens | |
0.045 | - |
(L-Pro-L-Pro-Gly)10 | recombinant type II enzyme tetramer | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | recombinant type II enzyme tetramer: Km 0.004 mM | Mus musculus | |
Fe2+ | type I enzyme tetramer: Km 0.004 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | expression of alpha-I and alpha-II subunits mRNAs | Mus musculus | - |
heart | - |
Homo sapiens | - |
heart | expression of enzyme alpha-I and alpha-II subunits mRNAs | Mus musculus | - |
lung | - |
Homo sapiens | - |
lung | expression of alpha-I and alpha-II subunits mRNAs | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2 | - |
Mus musculus | (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(Pro-Pro-Gly)10 + 2-oxoglutarate + O2 | - |
Homo sapiens | ? + succinate + CO2 | - |
? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Mus musculus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Homo sapiens | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | recombinant type II enzyme tetramer: Km 0.34 mM | Mus musculus | |
ascorbate | type I enzyme tetramer: Km 0.33 mM | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
poly (L-proline) | MW: 44000, type I enzyme tetramer | Homo sapiens | |
0.0005 | - |
poly (L-proline) | MW: 7000, type I enzyme tetramer | Homo sapiens | |
0.001 | - |
Pyridine 2,4-dicarboxylate | - |
Mus musculus | |
0.001 | - |
Pyridine 2,4-dicarboxylate | recombinant type II enzyme tetramer | Homo sapiens | |
0.002 | - |
Pyridine 2,4-dicarboxylate | type I enzyme tetramer | Homo sapiens | |
0.03 | - |
poly (L-proline) | - |
Mus musculus | |
0.03 | - |
poly (L-proline) | MW: 44000, recombinant type II enzyme tetramer | Homo sapiens | |
0.3 | - |
poly (L-proline) | - |
Mus musculus | |
0.3 | - |
poly (L-proline) | MW: 7000, recombinant type II enzyme tetramer | Homo sapiens |