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Literature summary for 1.14.11.17 extracted from
- Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313 (2000), J. Biol. Chem., 275, 31661-31667.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | required for activity | Pseudomonas putida |  |
| ascorbate | 3fold increase of activity at 0.2 mM | Pseudomonas putida | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.1 mM EDTA abolishes activity completely | Pseudomonas putida | |
| additional information | no reduction in enzyme activity is observed for sulfate concentrations up to 10 mM | Pseudomonas putida | |
| nonyl sulfate | inhibits at concentrations between 1 and 10 mM | Pseudomonas putida | |
| SDS | inhibits at concentrations between 1 and 10 mM | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for activity, highest activity in the presence of 0.1 mM | Pseudomonas putida | |
| additional information | Ni2+, Co2+, Mn2+, Cu2+, Zn2+, Mg2+, and Ca2+ have no stimulatory effect | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30250 | - |
4 * 30250, gel filtration | Pseudomonas putida |
| 32000 | - |
4 * 32000, SDS-PAGE | Pseudomonas putida |
| 121000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida S-313 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Resource-Q anion-exchange column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | hexanal + sulfite + succinate + CO2 | - |
? | |
| hexyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida S-313 | hexanal + sulfite + succinate + CO2 | - |
? | |
| additional information | the AtsK enzyme is not involved in the utilization of taurine as a sulfur source | Pseudomonas putida | ? | - |
? | |
| additional information | the AtsK enzyme is not involved in the utilization of taurine as a sulfur source | Pseudomonas putida S-313 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 32000, SDS-PAGE | Pseudomonas putida |
| homotetramer | 4 * 30250, gel filtration | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-ketoglutarate-dependent dioxygenase | - |
Pseudomonas putida |
| AtsK | - |
Pseudomonas putida |
| oxygenative alkylsulfatase | - |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in Tris-acetate buffer | Pseudomonas putida |
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