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Literature summary for 1.14.11.17 extracted from

  • Eichhorn, E.; Van der Ploeg, J.R.; Kertesz, M.A.; Leisinger, T.
    Characterization of alpha-ketoglutarate-dependent taurine dioxygenase from Escherichia coli (1997), J. Biol. Chem., 272, 23031-23036.
    View publication on PubMed
Search for more literature for 1.14.11.17

Activating Compound

Activating Compound Comment Organism Structure
ascorbate 50% increase in activity at 0.2-0.8 mM Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibits activity by 80-95% at 0.01-0.05 mM Escherichia coli
Cu2+ inhibits activity by 80-95% at 0.01-0.05 mM Escherichia coli
EDTA complete inactivation Escherichia coli
Zn2+ inhibits activity by 80-95% at 0.01-0.05 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 2-oxoglutarate
-
 Escherichia coli
0.055
-
 taurine
-
 Escherichia coli
0.145
-
 MOPS
-
 Escherichia coli
0.485
-
 1,3-dioxo-2-isoindolineethanesulfonic acid
-
 Escherichia coli
0.59
-
 pentanesulfonic acid
-
 Escherichia coli
1.49
-
 butanesulfonic acid
-
 Escherichia coli
1.51
-
 hexanesulfonic acid
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ maximal activation between 0.005 and 0.150 mM Escherichia coli
additional information Mg2+, Ca2+, Mn2+ or Ni2+ can not replace iron Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32410
-
 calculation from gene sequence Escherichia coli
37400
-
 estimated by SDS-PAGE Escherichia coli
81000
-
 gel filtration on Superose 6 and Superose 12 HR Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two-step purification from overexpressing Escherichia coli to apparent homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
 strain MC4100 grown in sulfate-free minimal medium containing 0.25 mM taurine as sulfur source Escherichia coli
1.64
-
 purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, phosphate buffer, 16% glycerol, 10 weeks, activity increases 4-fold Escherichia coli
-20°C, phosphate buffer, without glycerol, 3 weeks, more than 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dioxo-2-isoindolineethanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + ? + succinate + CO2
-
 ?
butanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + butanal + succinate + CO2
-
 ?
hexanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + hexanal + succinate + CO2
-
 ?
MOPS + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + ? + succinate + CO2
-
 ?
pentanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + pentanal + succinate + CO2
-
 ?
taurine + 2-oxoglutarate + O2 no substrates are methanesulfonic acid, ethanesulfonic acid, isethionic acid, 2-bromoethanesulfonic acid, L-cysteic acid, sulfosuccinate, 4-aminobenzenesulfonic acid, 2-(4-pyridyl)ethanesulfonic acid, N-phenyltaurine Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
 ?
taurine + alpha-ketoadipate + O2 alpha-ketoadipate is less active than 2-oxoglutarate, no activity with pyruvate, alpha-ketobutyrate, alpha-ketovalerate, alpha-ketocaproate, alpha-ketoisovalerate and oxalacetat Escherichia coli sulfite + aminoacetaldehyde + pentan-1,5-dioic acid + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37400, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 incubation at 30°C leads to rapid inactivation, effect is enhanced by ascorbate and not due to oxidation of the enzyme-bound ferrous iron Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Escherichia coli