Cloned (Comment) | Organism |
---|---|
gene MIOX1, transient recombinant overexpression of GFP-tagged OsMIOX, co-localizing with the endoplasmic reticulum marker, in Nicotiana benthamiana protoplasts, quantitative real-Time PCR enzyme expression analysis, recombinant expression of GST-tagged OsMIOX1 with MBP-tagged PeaT1 in Saccharomyces cerevisiae in the two-hybrid-system | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Oryza sativa | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryza sativa | interaction analysis of the enzyme amyoinositol oxygenase (OsMIOX) with PeaT1 elicitor from Alternaria tenuissima by yeast two-hybrid assay and pull-down assay, overview | ? | - |
? | |
myo-inositol + O2 | Oryza sativa | - |
D-glucuronate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q5Z8T3 | var. Nipponbare | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | interaction analysis of the enzyme amyoinositol oxygenase (OsMIOX) with PeaT1 elicitor from Alternaria tenuissima by yeast two-hybrid assay and pull-down assay, overview | Oryza sativa | ? | - |
? | |
myo-inositol + O2 | - |
Oryza sativa | D-glucuronate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amyoinositol oxygenase | - |
Oryza sativa |
Myo-inositol oxygenase | - |
Oryza sativa |
OsMIOX | - |
Oryza sativa |
Organism | Comment | Expression |
---|---|---|
Oryza sativa | OsMIOX enzyme expression is highly enhanced in rice plants overexpressing the Alternaria tenuissima PeaT1 gene during drought stress, the plants show enhanced drought stress tolerance and increased the survival rate following a drought treatment | up |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression of the PeaT1 elicitor gene from Alternaria tenuissima in Oryza sativa improves drought tolerance in rice plants via interaction with a myo-inositol oxygenase. In PeaT1-overexpressing (PeaT1OE) plants, abscisic acid and chlorophyll contents significantly increase, while the malondialdehyde (MDA) content decreases compared with the wild-type plants. Transcript levels of drought-responsive genes, including OsAM1, OsLP2, and OsDST, are prominently lower in the PeaT1OE plants. In contrast, expression levels of genes encoding positive drought stress regulators including OsSKIPa, OsCPK9, OsNAC9, OSEREBP1, and OsTPKb are upregulated in PeaT1OE plants. OsMIOX is highly expressed in PeaT1OE plants during the drought treatment | Oryza sativa |