Cloned (Comment) | Organism |
---|---|
gene miox, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein | Komagataella phaffii |
gene mMIOX, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440, recombinant coexpression of the enzyme in Escherichia coli with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | coexpression of the Mus musculus mMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein leads highly increased glutaric acid levels from myo-inositol in transfected Pichia pastoris cells. Production of glucaric acid with a fed-batch fermentation strategy, overview. Recombinant coexpression of the enzyme in Escherichia coli strains BL21(DE3) or MG1655 with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 leads to highly increased glutaric acid production, especially with addition of myo-inositol instead of glucose, production parameters, overview | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + O2 | Mus musculus | - |
D-glucuronate + H2O | - |
? | |
myo-inositol + O2 | Komagataella phaffii | - |
D-glucuronate + H2O | - |
? | |
myo-inositol + O2 | Komagataella phaffii GS115 | - |
D-glucuronate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella phaffii | C4QZH8 | i.e. Pichia pastoris | - |
Komagataella phaffii GS115 | C4QZH8 | i.e. Pichia pastoris | - |
Mus musculus | Q9QXN5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + O2 | - |
Mus musculus | D-glucuronate + H2O | - |
? | |
myo-inositol + O2 | - |
Komagataella phaffii | D-glucuronate + H2O | - |
? | |
myo-inositol + O2 | - |
Komagataella phaffii GS115 | D-glucuronate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MIOX | - |
Mus musculus |
MIOX | - |
Komagataella phaffii |
mMIOX | - |
Mus musculus |
Myo-inositol oxygenase | - |
Mus musculus |
Myo-inositol oxygenase | - |
Komagataella phaffii |
ppMIOX | - |
Komagataella phaffii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Komagataella phaffii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mus musculus |
8 | - |
assay at | Komagataella phaffii |
Organism | Comment | Expression |
---|---|---|
Komagataella phaffii | coexpression of the Pichia pastoris ppMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 does not lead to accumulation of glucaric acid from myo-inositol or increased enzyme activity of neither MIOX nor Udh | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is important in the glucaric acid synthetic pathway | Mus musculus |
metabolism | the enzyme is important in the glucaric acid synthetic pathway | Komagataella phaffii |