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Literature summary for 1.13.12.7 extracted from
- Tibetan firefly luciferase with low temperature adaptation (2017), Photochem. Photobiol., 93, 466-472 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 Star (DE3) | Pyrocoelia sp. RF-2016 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
D-firefly luciferin | pH 7.5, temperature not specified in the publication | Pyrocoelia sp. RF-2016 |  |
| 0.154 | - |
ATP | pH 7.5, temperature not specified in the publication | Pyrocoelia sp. RF-2016 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
SDS-PAGE | Pyrocoelia sp. RF-2016 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-firefly luciferin + O2 + ATP | Pyrocoelia sp. RF-2016 | - |
firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrocoelia sp. RF-2016 | A0A1J1DSP4 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| photophore | specifically expressed in the light organ | Pyrocoelia sp. RF-2016 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-firefly luciferin + O2 + ATP | - |
Pyrocoelia sp. RF-2016 | firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? | |
| D-firefly luciferin + O2 + ATP | the emission peak of this luciferase is 556 nm at pH 8.0, while it shifts to 606 nm at pH 6.0 | Pyrocoelia sp. RF-2016 | firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, SDS-PAGE | Pyrocoelia sp. RF-2016 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
the activity of Pyrocoelia sp. RF-2016 at 10°C is about 1.3times stronger than the Pyrocoelia pyralis luciferase activity at 25°C | Pyrocoelia sp. RF-2016 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
10 min, complete loss of activity | Pyrocoelia sp. RF-2016 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Pyrocoelia sp. RF-2016 |
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Release 2023.1 (January 2023)
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