Application | Comment | Organism |
---|---|---|
analysis | Renilla luciferase is a bioluminescent enzyme which is broadly used as a reporter protein in molecularbiosensors | Renilla reniformis |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Renilla reniformis |
Protein Variants | Comment | Organism |
---|---|---|
M185V/K189V/V267I | site-directed mutagenesis,compared with the native RLuc, mutant super RLuc has a higher turnover number, increased light output upon expression in Arabidopsis thaliana and enhanced half-life of photon emission, super RLuc is a blue light emitting luciferase | Renilla reniformis |
additional information | enzyme engineering of blue light emitting enzyme mutant super RLuc to construct a luciferase with desired light emission wavelength and thermostability, namely super RLuc8, which has a red-shifted spectrum and shows stable light emission. Super RLuc8 shows a 10fold increase in thermostability at 37°C after 20 min incubation, in comparison to the native enzyme. The optimum temperature of the mutant increases from 30 to 37°C. Molecular dynamics simulation analysis indicates that the increased thermostability is most probably caused by a better structural compactness and more local rigidity in the regions out of the emitter site. The mutant super RLuc8 shows increased activity compared t the wild-type. Molecular dynamics simulation, overview | Renilla reniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00293 | - |
coelenterazine | pH 7.2, 37°C, recombinant engineered mutant super RLuc 8 | Renilla reniformis | |
0.00997 | - |
coelenterazine | pH 7.2, 37°C, recombinant wild-type enzyme | Renilla reniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | Renilla reniformis | - |
coelenteramide + CO2 + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Renilla reniformis | P27652 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Renilla reniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | - |
Renilla reniformis | coelenteramide + CO2 + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, modified recombinant His-tagged enzyme super RLuc 8, SDS-PAGE | Renilla reniformis |
Synonyms | Comment | Organism |
---|---|---|
Renilla luciferase | - |
Renilla reniformis |
RLuc | - |
Renilla reniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
wild-type enzyme | Renilla reniformis |
36 | 37 | engineered enzyme mutant super RLuc8 | Renilla reniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 7.8, 60 min, 4 and 10fold increases in recombinant His-tagged engineered super RLuc8 thermostability are observed at 37°C after 10 and 20 min incubation, respectively, in comparison to the recombinant His-tagged wild-type enzyme. While the wild-type enzyme is completely inactivated after 30 min, the mutant enzyme retains about 30% of its initial activity | Renilla reniformis |
45 | - |
pH 7.8, the activity of purified recombinant His-tagged engineered mutant superRLuc8 remains around 60% after incubation at 45°C, while wild-type recombinant His-tagged RLuc loses approximately 82% of its initial activity at the same temperature | Renilla reniformis |
General Information | Comment | Organism |
---|---|---|
additional information | molecular dynamic simulation of the native RLuc based on PDB ID 2PSF, which is the crystal structure of the mutant RLuc luciferase, overview | Renilla reniformis |