Literature summary for 1.13.11.8 extracted from

Tsagogiannis, E.; Vandera, E.; Primikyri, A.; Asimakoula, S.; Tzakos, A.G.; Gerothanassis, I.P.; Koukkou, A.I.
Characterization of protocatechuate 4,5-dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and in situ reaction monitoring in the NMR tube (2021), Int. J. Mol. Sci., 22, 9647 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudarthrobacter phenanthrenivorans

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	strong inhibition	Pseudarthrobacter phenanthrenivorans
Mn2+	strong inhibition	Pseudarthrobacter phenanthrenivorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	3,4-dihydroxybenzoate	pH 9.5, 20°C, recombinant enzyme	Pseudarthrobacter phenanthrenivorans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	more than a 6-fold increase in specific activity after incubation of the enzyme with 0.2 mM Fe(II) and L-ascorbic acid	Pseudarthrobacter phenanthrenivorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	SDS-PAGE	Pseudarthrobacter phenanthrenivorans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3,4-dihydroxybenzoate + O2	Pseudarthrobacter phenanthrenivorans	-	4-carboxy-2-hydroxymuconate semialdehyde	-	?
3,4-dihydroxybenzoate + O2	Pseudarthrobacter phenanthrenivorans Sphe3	-	4-carboxy-2-hydroxymuconate semialdehyde	-	?

Organic Solvent Stability

Organic Solvent	Comment	Organism
DMSO	1% (v/v), stabilizes	Pseudarthrobacter phenanthrenivorans

Organism

Organism	UniProt	Comment	Textmining
Pseudarthrobacter phenanthrenivorans	F0MCP7	-	-
Pseudarthrobacter phenanthrenivorans Sphe3	F0MCP7	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudarthrobacter phenanthrenivorans

Storage Stability

Storage Stability	Organism
-80 °C, in the presence of 10% (v/v) glycerol, purified enzyme maintains 93% of its activity for up to at least 4 months	Pseudarthrobacter phenanthrenivorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydroxybenzoate + O2	-	Pseudarthrobacter phenanthrenivorans	4-carboxy-2-hydroxymuconate semialdehyde	-	?
3,4-dihydroxybenzoate + O2	-	Pseudarthrobacter phenanthrenivorans Sphe3	4-carboxy-2-hydroxymuconate semialdehyde	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 45000, SDS-PAGE	Pseudarthrobacter phenanthrenivorans

Synonyms

Synonyms	Comment	Organism
PcaA	-	Pseudarthrobacter phenanthrenivorans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	-	Pseudarthrobacter phenanthrenivorans

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	45	10°C: about 20% of maximal activity, 45°C: about 20% of maximal activity	Pseudarthrobacter phenanthrenivorans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Pseudarthrobacter phenanthrenivorans

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10.5	active within pH 5.5 and 10.5, no activity at pH 5.0, 1nd 11.0	Pseudarthrobacter phenanthrenivorans

Expression

Organism	Comment	Expression
Pseudarthrobacter phenanthrenivorans	the expression of pcaA gene is induced in cells grown on phenanthrene or 3,4-dihydroxybenzoate showing up to 139- and 304-fold change increase in mRNA expression levels in the presence of phenanthrene and 3,4-dihydroxybenzoate, respectively, when compared to glucose	up

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Release 2023.1 (January 2023)