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Literature summary for 1.13.11.66 extracted from

  • Mancini, S.; Abicht, H.K.; Gonskikh, Y.; Solioz, M.
    A copper-induced quinone degradation pathway provides protection against combined copper/quinone stress in Lactococcus lactis IL1403 (2015), Mol. Microbiol., 95, 645-659 .
    View publication on PubMed
Search for more literature for 1.13.11.66

Cloned(Commentary)

Cloned (Comment) Organism
gene yaiA, encoded in the the yahCD-yaiAB operon, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli. YaiA activity could be detected in crude Escherichia coli cytoplasmic extract overexpressing Lactcoccus lactis YaiA from a plasmid Lactococcus lactis

General Stability

General Stability Organism
4-hydrobenzoate, the competitive inhibitor of the heterotetrameric HapCD hydroquinone dioxygenase of Pseudomonas fluorescens ACB, stabilizes the enzyme Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
additional information 4-hydrobenzoate, the competitive inhibitor of the heterotetrameric HapCD hydroquinone dioxygenase of Pseudomonas fluorescens ACB, stabilizes the enzyme Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 2-methylhydroquinone pH 6.4, 30°C, recombinant enzyme Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
copper copper content is 0.12 atoms per monomer Lactococcus lactis
Iron iron content is 0.96 atoms per monomer Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methylhydroquinone + O2 Lactococcus lactis
-
 ?
-
 ?
2-methylhydroquinone + O2 Lactococcus lactis IL1403
-
 ?
-
 ?
benzene-1,4-diol + O2 Lactococcus lactis
-
 (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
benzene-1,4-diol + O2 Lactococcus lactis IL1403
-
 (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
additional information Lactococcus lactis YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA ?
-
 ?
additional information Lactococcus lactis IL1403 YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis IL1403
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag removal by rTEV protease cleavage, over 95% purity. No enzyme activity can be detected with purified YaiA, even when it is purified in the presence of 4-hydrobenzoate Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dimethylhydroquinone + O2
-
 Lactococcus lactis 2-methylmaleylacetone
-
 ?
2,5-dimethylhydroquinone + O2
-
 Lactococcus lactis IL1403 2-methylmaleylacetone
-
 ?
2-methylhydroquinone + O2
-
 Lactococcus lactis ?
-
 ?
2-methylhydroquinone + O2
-
 Lactococcus lactis IL1403 ?
-
 ?
2-methylhydroquinone + O2
-
 Lactococcus lactis maleylacetone
-
 ?
benzene-1,4-diol + O2
-
 Lactococcus lactis (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
benzene-1,4-diol + O2
-
 Lactococcus lactis IL1403 (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
additional information YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA Lactococcus lactis ?
-
 ?
additional information 2-methylhydroquinone is a slightly better substrate than unsubstituted hydroquinone Lactococcus lactis ?
-
 ?
additional information YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA Lactococcus lactis IL1403 ?
-
 ?
additional information 2-methylhydroquinone is a slightly better substrate than unsubstituted hydroquinone Lactococcus lactis IL1403 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43300, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
hydroquinone dioxygenase
-
 Lactococcus lactis
YaiA
-
 Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 with substrate 2-methylhydroquinone Lactococcus lactis

Expression

Organism Comment Expression
Lactococcus lactis the the yahCD-yaiAB operon is induced by copper but not by quinones up

General Information

General Information Comment Organism
physiological function the enzyme YaiA plays a key role in alleviating quinone/copper stress. The yahCD-yaiAB operon of Lactococcus lactis IL1403 provides resistance to combined copper/quinone stress. The operon is under the control of CopR, which also regulates expression of the copRZA copper resistance operon as well as other Lactococcus lactis genes. Expression of the yahCD-yaiAB operon is induced by copper but not by quinones. Copper induction of the yahCD-yaiAB operon offers protection to copper/quinone toxicity and can provide a growth advantage to Lactococcus lactis in some environments. Regulation of quinone degradation by CopR thus appears to be a hallmark of Lactococci. The conversion of hydroquinone to 4-hydroxymuconic semialdehyde by the YaiA dioxygenase appears to be too slow for acute detoxification, but probably serves as long-term remedy by degrading the hydroquinone pool, which constitutes a reservoir for the formation of toxic pbenzoquinones by spontaneous oxidation that is greatly accelerated by copper Lactococcus lactis