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Literature summary for 1.13.11.66 extracted from

  • Xun, L.; Bohuslavek, J.; Cai, M.
    Characterization of 2,6-dichloro-p-hydroquinone 1,2-dioxygenase (PcpA) of Sphingomonas chlorophenolica ATCC 39723 (1999), Biochem. Biophys. Res. Commun., 266, 322-325.
    View publication on PubMed
Search for more literature for 1.13.11.66

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingobium chlorophenolicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0135
-
 2,6-dichloro-p-hydroquinone pH 6.7, 22°C Sphingobium chlorophenolicum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe2+ is absolutely required, Km value 0.0046 mM Sphingobium chlorophenolicum

Organism

Organism UniProt Comment Textmining
Sphingobium chlorophenolicum Q9ZBB0 ATCC 39723
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dichloro-p-hydroquinone + O2
-
 Sphingobium chlorophenolicum 2-chloromaleylacetate + Cl-
-
 ?
additional information no substrates: 2,5-dichloro-p-hydroquinone, 6-chlorohydroxyquinol, hydroxyquinol, 2-chloro-p-hydroquinone, catechol, and 4-fluorocatechol Sphingobium chlorophenolicum ?
-
 ?

Synonyms

Synonyms Comment Organism
PcpA
-
 Sphingobium chlorophenolicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 2,6-dichloro-p-hydroquinone pH 6.7, 22°C Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 optimal in 20 mM potassium phosphate buffer Sphingobium chlorophenolicum

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8
-
 Sphingobium chlorophenolicum