Cloned (Comment) | Organism |
---|---|
gene BCMO1, expression of enzyme with C-terminal C-terminal TEV-, His6- and 1D4-tags in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | at 0.245% | Homo sapiens | |
hexaethylene glycol monooctyl ether | at 0.195-0.78% | Homo sapiens | |
additional information | addition of detergents fails to increase BCMO1 enzymatic activity, but short chain aliphatic detergents such as C8E4 and C8E6 decrease enzymatic activity probably by interacting with the substrate binding site | Homo sapiens | |
n-octyl-beta-D-thioglucopyranoside | at 0.56% | Homo sapiens | |
tetraethylene glycol monooctyl ether | at 0.125-0.5% | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Homo sapiens | |
0.0137 | 0.0148 | beta-carotene | recombinant enzyme, pH 7.5, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
additional information | determination of BCMO1 subcellular localization in mouse liver, overview | Mus musculus | - |
- |
soluble | BCMO1 is soluble and its enzymatic activity is not dependent on a membrane-like environment | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme is a non-heme iron oxygenase | Mus musculus | |
Fe2+ | the enzyme is a non-heme iron oxygenase | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | Mus musculus | - |
2 all-trans-retinal | - |
? | |
beta-carotene + O2 | Homo sapiens | - |
2 all-trans-retinal | - |
? | |
beta-carotene + O2 | Mus musculus C57/BL6 / 129 Sv | - |
2 all-trans-retinal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HAY6 | gene Bcmo1 | - |
Mus musculus | Q9JJS6 | gene Bcmo1 | - |
Mus musculus C57/BL6 / 129 Sv | Q9JJS6 | gene Bcmo1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially from Stellate cell cytosol | Mus musculus |
recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags from Spodoptera frugiperda Sf9 cells by Co2+ affinity chromatography and gel filtration in absence of detergent | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
stellate cell | high enzyme expression | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | - |
Mus musculus | 2 all-trans-retinal | - |
? | |
beta-carotene + O2 | - |
Homo sapiens | 2 all-trans-retinal | - |
? | |
beta-carotene + O2 | - |
Mus musculus C57/BL6 / 129 Sv | 2 all-trans-retinal | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Mus musculus |
monomer | 1 x 65200, recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Bcmo1 | - |
Mus musculus |
Bcmo1 | - |
Homo sapiens |
beta,beta-carotene-15,15'-monooxygenase | - |
Mus musculus |
beta,beta-carotene-15,15'-monooxygenase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | 8.5 | beta-carotene | recombinant enzyme, pH 7.5, temperature not specified in the publication | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant BCMO1 does not require cofactors | Homo sapiens |