Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Gold (DE3) cells | Cyanothece sp. PCC 7425 |
Crystallization (Comment) | Organism |
---|---|
mutant enzymes Q74V and Q74E, sitting drop vapor diffusion method, using 0.1 M MES (pH 6.5), 0.15 M MgSO4, 28% (w/v) polyethylene glycol 3350, and 3% (v/v) glycerol | Cyanothece sp. PCC 7425 |
Protein Variants | Comment | Organism |
---|---|---|
Q74E | the catalytic efficiency (kcat/KM) of chlorite degradation of the variant is similar to that of the wild type protein | Cyanothece sp. PCC 7425 |
Q74V | the catalytic efficiency (kcat/KM) of chlorite degradation of the variant is similar to that of the wild type protein | Cyanothece sp. PCC 7425 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chlorite | above 0.1 mM chlorite (5000fold excess) irreversible inactivation is observed | Cyanothece sp. PCC 7425 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0188 | - |
Chlorite | mutant enzyme Q74V, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.0193 | - |
Chlorite | mutant enzyme Q74V, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.021 | - |
Chlorite | mutant enzyme Q74E, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.0432 | - |
Chlorite | wild type enzyme, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.0478 | - |
Chlorite | mutant enzyme Q74V, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.0538 | - |
Chlorite | mutant enzyme Q74E, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.0592 | - |
Chlorite | wild type enzyme, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.1271 | - |
Chlorite | wild type enzyme, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 | |
0.327 | - |
Chlorite | mutant enzyme Q74E, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | Cyanothece sp. PCC 7425 | - |
chloride + O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyanothece sp. PCC 7425 | B8HNS6 | - |
- |
Purification (Comment) | Organism |
---|---|
His-trap affinity column chromatography and Superdex 200 gel filtration | Cyanothece sp. PCC 7425 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | - |
Cyanothece sp. PCC 7425 | chloride + O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chlorite dismutase | - |
Cyanothece sp. PCC 7425 |
CLD | - |
Cyanothece sp. PCC 7425 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.91 | - |
Chlorite | mutant enzyme Q74E, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
33.4 | - |
Chlorite | wild type enzyme, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
37.2 | - |
Chlorite | mutant enzyme Q74E, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
43 | - |
Chlorite | mutant enzyme Q74V, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
50.8 | - |
Chlorite | mutant enzyme Q74V, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
60 | - |
Chlorite | wild type enzyme, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
280.2 | - |
Chlorite | mutant enzyme Q74V, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 | |
475.8 | - |
Chlorite | wild type enzyme, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 | |
977.4 | - |
Chlorite | mutant enzyme Q74E, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | - |
Cyanothece sp. PCC 7425 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
560 | - |
Chlorite | wild type enzyme, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
690 | - |
Chlorite | mutant enzyme Q74E, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
900 | - |
Chlorite | mutant enzyme Q74E, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
1400 | - |
Chlorite | wild type enzyme, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
2200 | - |
Chlorite | mutant enzyme Q74V, at pH 9.0 and 30°C | Cyanothece sp. PCC 7425 | |
2700 | - |
Chlorite | mutant enzyme Q74V, at pH 7.0 and 30°C | Cyanothece sp. PCC 7425 | |
3000 | - |
Chlorite | mutant enzyme Q74E, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 | |
3700 | - |
Chlorite | wild type enzyme, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 | |
5900 | - |
Chlorite | mutant enzyme Q74V, at pH 5.0 and 30°C | Cyanothece sp. PCC 7425 |