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Literature summary for 1.13.11.33 extracted from

  • Kuehn, H.; Barnett, J.; Grunberger, D.; Baecker, P.; Chow, J.; Nguyen, B.; Bursztyn-Pettegrew, H.; Chan, H.; Sigal, E.
    Overexpression, purification and characterization of human recombinant 15-lipoxygenase (1993), Biochim. Biophys. Acta, 1169, 80-89.
    View publication on PubMed
Search for more literature for 1.13.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expression to high levels, approximately 20% of cellular protein, in a baculovirus/insect cell expression system Homo sapiens

General Stability

General Stability Organism
repeated freeze thawing especially in the absence of gylcerol results in 60% reduction of specific activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,8,11,14-Eicosatetraynoic acid inhibits reaction of linoleic acid Homo sapiens
additional information the enzyme undergoes suicidal inactivation, during fatty acid oxygenation Homo sapiens
nordihydroguaiaretic acid
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 linoleic acid
-
 Homo sapiens
0.012
-
 arachidonic acid
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ addition in a molar excess, more than 50:1 purified protein:Fe2+, leads to a 3fold increase in the specific activity Homo sapiens
Iron the recombinant enzyme contains a variable amount of iron, often as low as 0.2 mol per mol of enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 x * 72000, SDS-PAGE Homo sapiens
74727
-
 x * 74727, electrospray mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 5% carbohydrate Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, 5% glycerol, stable for more than 6 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate formation of 15S-hydroxyeicosatetraenoic acid and 12S-hydroxyeicosatetraenoic acid in a ratio of 12:1, double oxygenation products 14R,15S-dihydroxyeicosatetraenoic acid and various 8,15-dihydroxyeicosatetraenoic acids are also produced ?
linoleic acid + O2
-
 Homo sapiens 13-hydroxylinoleic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE Homo sapiens
? x * 74727, electrospray mass spectrometry Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens